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Literature summary for 3.2.1.51 extracted from

  • Endo, T.; Tsukada, T.; Hiraiwa, M.; Uda, Y.; Kobata, A.
    Purification and characterization of an alpha-L-fucosidase from Pomacea canaliculata (1993), Arch. Biochem. Biophys., 302, 152-160.
    View publication on PubMed
Search for more literature for 3.2.1.51

Application

Application Comment Organism
analysis studies of structures and functions of glycoconjugates and oligosaccharides due to unique substrate specificity Pomacea canaliculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Pomacea canaliculata
1.18
-
 2'-fucosyllactitol
-
 Pomacea canaliculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 4 * 60000, SDS-PAGE Pomacea canaliculata
260000
-
 gel filtration Pomacea canaliculata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-fucosyllactitol + H2O Pomacea canaliculata
-
 alpha-L-fucose + lactitol
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O Pomacea canaliculata lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Pomacea canaliculata lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Pomacea canaliculata hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O Pomacea canaliculata lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O Pomacea canaliculata very slow hydrolysis, NaBH4-reduced oligosaccharide Gal-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O Pomacea canaliculata
-
 [Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
 ?

Organism

Organism UniProt Comment Textmining
Pomacea canaliculata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Pomacea canaliculata

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Pomacea canaliculata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3
-
-
 Pomacea canaliculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fucosyllactitol + H2O
-
 Pomacea canaliculata alpha-L-fucose + lactitol
-
 ?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Pomacea canaliculata 4-nitrophenol + alpha-L-fucose
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide Pomacea canaliculata Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide Pomacea canaliculata Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O NaBH4-reduced oligosaccharide, hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage Pomacea canaliculata Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage Pomacea canaliculata Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide Pomacea canaliculata alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide Pomacea canaliculata alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O NaBH4-reduced oligosaccharide, very slow hydrolysis Pomacea canaliculata Gal-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O very slow hydrolysis, NaBH4-reduced oligosaccharide Pomacea canaliculata Gal-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
-
 Pomacea canaliculata [Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
 ?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O subscript OT is used to indicate NaBH4-reduced oligosaccharide Pomacea canaliculata [Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, SDS-PAGE Pomacea canaliculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
 two pH-optima Pomacea canaliculata
5
-
 two pH-optima Pomacea canaliculata