General Stability | Organism |
---|---|
increased half-life to 23 h at 37°C and stability to repeated freezing and thawing by addition of 0.5 mg/ml bovine serum albumin | Cricetulus griseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-L-fucose | competitive inhibition, Ki: 0.065 mM | Cricetulus griseus | |
deoxyfuconojirimycin | competitive inhibition, Ki: 0.000024 mM | Cricetulus griseus | |
fucosylamine | competitive inhibition, Ki: 0.014 mM | Cricetulus griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | at pH 7.0 | Cricetulus griseus | |
0.072 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | at pH 5.0 | Cricetulus griseus | |
1.02 | - |
gp120 oligosaccharide | at pH 5.0 | Cricetulus griseus | |
1.75 | - |
Fuc-alpha-(1-6)-GlcNAc | at pH 5.0 | Cricetulus griseus | |
1.79 | - |
2'-fucosyllactose | at pH 5.0 | Cricetulus griseus | |
1.9 | - |
3'-fucosyllactose | at pH 5.0 | Cricetulus griseus | |
10.6 | - |
2'-fucosyllactose | at pH 7.0 | Cricetulus griseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | normally a lysosomal glycoprotein, released by secretion and/or cell lysis | Cricetulus griseus | - |
- |
lysosome | - |
Cricetulus griseus | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
SDS-PAGE | Cricetulus griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | Cricetulus griseus | 2'-fucosyllactose | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O | Cricetulus griseus | low activity | alpha-D-fucose + lactose | - |
? | |
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O | Cricetulus griseus | 3-fucosyllactose | alpha-D-fucose + lactose | - |
? | |
Fuc-alpha-(1-6)-GlcNAc + H2O | Cricetulus griseus | - |
alpha-L-fucose + N-acetyl-D-glucosamine | - |
? | |
gp120 oligosaccharides + H2O | Cricetulus griseus | released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates | alpha-L-fucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
Chinese hamster ovary cells | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Cricetulus griseus |
Purification (Comment) | Organism |
---|---|
to homogeneity, affinity chromatography | Cricetulus griseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Cricetulus griseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
64.8 | - |
- |
Cricetulus griseus |
Storage Stability | Organism |
---|---|
-20°C, 0.5 mg/ml bovine serum albumin, 6 months | Cricetulus griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-fucosyllactose + H2O | - |
Cricetulus griseus | alpha-L-fucose + ? | - |
? | |
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O | - |
Cricetulus griseus | 4-methylumbelliferone + alpha-L-fucose | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | 2'-fucosyllactose | Cricetulus griseus | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O | low activity | Cricetulus griseus | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O | 3-fucosyllactose | Cricetulus griseus | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O | low activity | Cricetulus griseus | alpha-D-fucose + lactose | - |
? | |
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O | 3-fucosyllactose | Cricetulus griseus | alpha-D-fucose + lactose | - |
? | |
Fuc-alpha-(1-6)-GlcNAc + H2O | - |
Cricetulus griseus | alpha-L-fucose + N-acetyl-D-glucosamine | - |
? | |
gp120 oligosaccharide + H2O | - |
Cricetulus griseus | alpha-L-fucose + ? | - |
? | |
gp120 oligosaccharides + H2O | released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates | Cricetulus griseus | alpha-L-fucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 51000, SDS-PAGE | Cricetulus griseus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life: 1 h | Cricetulus griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
optimal hydrolysis rate for 2'-fucosyllactose and Fuc-alpha-(1-6)-GlcNAc | Cricetulus griseus |
7 | - |
optimal hydrolysis rate for 4-methylumbelliferyl-alpha-L-fucopyranoside | Cricetulus griseus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000024 | - |
deoxyfuconojirimycin | competitive inhibition | Cricetulus griseus | |
0.014 | - |
fucosylamine | competitive inhibition | Cricetulus griseus |