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Literature summary for 3.2.1.51 extracted from

  • Gramer, M.J.; Schaffer, D.V.; Sliwkowski, M.B.; Goochee, C.F.
    Purification and characterization of alpha-L-fucosidase from Chinese hamster ovary cell culture supernatant (1994), Glycobiology, 4, 611-616.
    View publication on PubMed

General Stability

General Stability Organism
increased half-life to 23 h at 37°C and stability to repeated freezing and thawing by addition of 0.5 mg/ml bovine serum albumin Cricetulus griseus

Inhibitors

Inhibitors Comment Organism Structure
alpha-L-fucose competitive inhibition, Ki: 0.065 mM Cricetulus griseus
deoxyfuconojirimycin competitive inhibition, Ki: 0.000024 mM Cricetulus griseus
fucosylamine competitive inhibition, Ki: 0.014 mM Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
4-methylumbelliferyl-alpha-L-fucopyranoside at pH 7.0 Cricetulus griseus
0.072
-
4-methylumbelliferyl-alpha-L-fucopyranoside at pH 5.0 Cricetulus griseus
1.02
-
gp120 oligosaccharide at pH 5.0 Cricetulus griseus
1.75
-
Fuc-alpha-(1-6)-GlcNAc at pH 5.0 Cricetulus griseus
1.79
-
2'-fucosyllactose at pH 5.0 Cricetulus griseus
1.9
-
3'-fucosyllactose at pH 5.0 Cricetulus griseus
10.6
-
2'-fucosyllactose at pH 7.0 Cricetulus griseus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular normally a lysosomal glycoprotein, released by secretion and/or cell lysis Cricetulus griseus
-
-
lysosome
-
Cricetulus griseus 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
SDS-PAGE Cricetulus griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Cricetulus griseus 2'-fucosyllactose alpha-L-fucose + lactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O Cricetulus griseus low activity alpha-D-fucose + lactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O Cricetulus griseus 3-fucosyllactose alpha-D-fucose + lactose
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O Cricetulus griseus
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
?
gp120 oligosaccharides + H2O Cricetulus griseus released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates alpha-L-fucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
Chinese hamster ovary cells
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Cricetulus griseus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, affinity chromatography Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
64.8
-
-
Cricetulus griseus

Storage Stability

Storage Stability Organism
-20°C, 0.5 mg/ml bovine serum albumin, 6 months Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-fucosyllactose + H2O
-
Cricetulus griseus alpha-L-fucose + ?
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
Cricetulus griseus 4-methylumbelliferone + alpha-L-fucose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O 2'-fucosyllactose Cricetulus griseus alpha-L-fucose + lactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O low activity Cricetulus griseus alpha-L-fucose + lactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O 3-fucosyllactose Cricetulus griseus alpha-L-fucose + lactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O low activity Cricetulus griseus alpha-D-fucose + lactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O 3-fucosyllactose Cricetulus griseus alpha-D-fucose + lactose
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
-
Cricetulus griseus alpha-L-fucose + N-acetyl-D-glucosamine
-
?
gp120 oligosaccharide + H2O
-
Cricetulus griseus alpha-L-fucose + ?
-
?
gp120 oligosaccharides + H2O released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates Cricetulus griseus alpha-L-fucose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 51000, SDS-PAGE Cricetulus griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
half-life: 1 h Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
optimal hydrolysis rate for 2'-fucosyllactose and Fuc-alpha-(1-6)-GlcNAc Cricetulus griseus
7
-
optimal hydrolysis rate for 4-methylumbelliferyl-alpha-L-fucopyranoside Cricetulus griseus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000024
-
deoxyfuconojirimycin competitive inhibition Cricetulus griseus
0.014
-
fucosylamine competitive inhibition Cricetulus griseus