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Literature summary for 3.2.1.50 extracted from

  • Ficko-Blean, E.; Stubbs, K.A.; Nemirovsky, O.; Vocadlo, D.J.; Boraston, A.B.
    Structural and mechanistic insight into the basis of mucopolysaccharidosis IIIB (2008), Proc. Natl. Acad. Sci. USA, 105, 6560-6565.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.50

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridium perfringens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Clostridium perfringens

Protein Variants

Protein Variants Comment Organism
E601A mutation in the active site, lack of enzymatic activity Clostridium perfringens
mutagenesis of Glu-483 mutation in the active site, less enzymatic activity Clostridium perfringens

Inhibitors

Inhibitors Comment Organism Structure
2-acetamido-1,2-dideoxynojirimycin
-
 Clostridium perfringens
6-acetamido-6-deoxycastanospermine 6AcCAS Clostridium perfringens
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
-
 Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.74
-
 2,4-dinitrophenyl-alpha-N-acetyl-D-glucosaminide pH 7.3 Clostridium perfringens
1.1
-
 4-nitrophenyl-alpha-N-acetyl-D-glucosaminide pH 7.3 Clostridium perfringens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Clostridium perfringens 5764
-

Organism

Organism UniProt Comment Textmining
Clostridium perfringens A0A0H2YU91
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium perfringens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl-alpha-N-acetyl-D-glucosaminide + H2O 25°C Clostridium perfringens 2,4-dinitrophenol + N-acetyl-D-glucosamine
-
 ?
4-nitrophenyl-alpha-N-acetyl-D-glucosaminide + H2O 25°C Clostridium perfringens 4-nitrophenol + N-acetyl-alpha-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
alpha-N-acetylglucosaminidase
-
 Clostridium perfringens
exo-glycosidase
-
 Clostridium perfringens
NAGLU
-
 Clostridium perfringens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
 4-nitrophenyl N-acetyl-alpha-D-glucosaminopyranoside pH 7.3 Clostridium perfringens
8.5
-
 2,4-dinitrophenyl-alpha-N-acetyl-D-glucosaminide pH 7.3 Clostridium perfringens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Clostridium perfringens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000036
-
 2-acetamido-1,2-dideoxynojirimycin
-
 Clostridium perfringens
0.000092
-
 6-acetamido-6-deoxycastanospermine
-
 Clostridium perfringens
0.0062
-
 O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
-
 Clostridium perfringens