Application | Comment | Organism |
---|---|---|
medicine | the use of secreted enzyme in future enzyme and gene replacement therapy protocols will by severely limited due to its small degree of mannose-6-phosphorylation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in CHO-K1 cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0166 | - |
O-(alpha-acetamido-2-deoxy-D-glucopyranosyl)-(1-3)-[L-6,3H]idoronic acid | pH 4.5, 37°C, with bovine serum albumin | Homo sapiens | |
1.07 | - |
4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside | pH 4.5, 37°C, without bovine serum albumin | Homo sapiens | |
5.34 | - |
4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside | pH 4.5, 37°C, with bovine serum albumin | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
x * 79000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE | Homo sapiens |
89000 | - |
x * 89000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern | Homo sapiens |
phosphoprotein | the specific phosphorylation of recombinant enzyme secreted from transfected CHO cells is significantly lower when compared with a control lysosomal enzyme | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | of cells CHO cells stable transfected | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.057 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside + H2O | - |
Homo sapiens | ? | - |
? | |
O-(alpha-acetamido-2-deoxy-D-glucopyranosyl)-(1-3)-[L-6,3H]idoronic acid + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE | Homo sapiens |
? | x * 89000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NAGLU | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
reaction 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside | Homo sapiens |