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Literature summary for 3.2.1.50 extracted from
- Purification and characterization of recombinant human alpha-N-acetylglucosaminidase secreted by chinese hamster ovary cells (2000), Protein Expr. Purif., 19, 202-211.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Chinese hamster ovary cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-acetamido-1,2-dideoxynojirimycin | competitive inhibition | Homo sapiens |  |
| 6-acetamido-6-deoxycastanospermine | competitive inhibition | Homo sapiens | |
| iodoacetate | complete inhibition at 2.5 mM | Homo sapiens | |
| p-chloromercuribenzoate | complete inhibition at 0.005 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
methylumbelliferyl-N-acetyl-alpha-D-glucosaminide | purified enzyme | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | complete inhibition at 0.0015 mM | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
SDS-PAGE | Homo sapiens |
| 90000 | - |
2 * 90000, monomer of 90000 and dimer in a ratio 1:2, gel filtration | Homo sapiens |
| 90000 | 180000 | monomer of 90000 and dimer of 180000 in the ratio 1:2, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (GlcNAcalpha(1-4))n + H2O | Homo sapiens | involved in the degradation of heparin and heparan sulfate | (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | naturally occuring enzyme contains mannose-6-phosphate | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ovary | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.58 | - |
purified enzyme, methylumbelliferyl-N-acetyl-alpha-D-glucosaminide as substrate | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10 mM sodium phosphate buffer, pH 7.2, 150 mM NaCl, 1 year, no significant loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (GlcNAcalpha(1-4))n + H2O | involved in the degradation of heparin and heparan sulfate | Homo sapiens | (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | - |
? | |
| methylumbelliferyl-N-acetyl-alpha-D-glucosaminide + H2O | - |
Homo sapiens | methylumbelliferone + N-acetyl-alpha-D-glucosamine | - |
? | |
| uridine-5'-diphospho-N-acetyl-alpha-D-glucosamine | - |
Homo sapiens | uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 90000, monomer of 90000 and dimer in a ratio 1:2, gel filtration | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | complete loss of activity by heating at 70°C for 4 min | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 8.1 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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