Literature summary for 3.2.1.50 extracted from

Sasaki, T.; Sukegawa, K.; Masue, M.; Fukuda, S.; Tomatsu, S.; Orii, T.
Purification and partial characterization of alpha-N-acetylglucosaminidase from human liver (1991), J. Biochem., 110, 842-846.

Search for more literature for 3.2.1.50

Inhibitors

Inhibitors	Comment	Organism	Structure
mouse anti-serum	raised in BALB/c mice, complete inhibition at pH 7.5	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.13	0.2	p-nitrophenyl-alpha-N-acetylglucosaminide	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	SDS-PAGE	Homo sapiens
300000	307000	gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(GlcNAcalpha(1-4))n + H2O	Homo sapiens	involved in the degradation of heparin and heparan sulfate	(GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(GlcNAcalpha(1-4))n + H2O	involved in the degradation of heparin and heparan sulfate	Homo sapiens	(GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose	-	?
p-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O	-	Homo sapiens	p-nitrophenol + N-acetyl-alpha-D-glucosamine	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	70	stable at 50°C for 1 h, at 55°C loss of 17%, at 60°C loss of 77% of activity	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	p-nitrophenyl-alpha-N-acetylglucosaminide	Homo sapiens

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6.5	8.5	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)