Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 10 mM, 12% residual activity | Aspergillus niger | |
additional information | not inhibitory: Ca2+, Mg2+, Mn2+, Ag+ | Aspergillus niger | |
Pb2+ | 10 mM, 30% residual activity | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
2-nitrophenyl-alpha-N-acetylgalactosaminide | pH 3.0, 35°C | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
6 * 76000, SDS-PAGE | Aspergillus niger |
440000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
best producer of alpha-N-acetylgalactosaminase | - |
Aspergillus niger CCIM K2 | - |
best producer of alpha-N-acetylgalactosaminase | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme may be deglycosylated without loss of activity | Aspergillus niger |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.5 | - |
pH 3.0, 35°C | Aspergillus niger |
Storage Stability | Organism |
---|---|
-20°C, about 5% loss of activity | Aspergillus niger |
4°C, pH 1.5-4.0, stable for several months | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O | enzyme acts with retention of the anomeric configuration | Aspergillus niger | 2-nitrophenol + alpha-N-acetylgalactosamine | - |
? | |
2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O | enzyme acts with retention of the anomeric configuration | Aspergillus niger CCIM K2 | 2-nitrophenol + alpha-N-acetylgalactosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 76000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable for at least 3 days | Aspergillus niger |
55 | - |
5 h, 50% loss of activity | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
1.8 | - |
- |
Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1.5 | 4 | - |
Aspergillus niger |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | isoelectric focusing | - |
4.8 |