Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.49 extracted from

  • Ohta, M.; Ohnishi, T.; Ioannou, Y.A.; Hodgson, M.E.; Matsuura, F.; Desnick, R.J.
    Human alpha-N-acetylgalactosaminidase: site occupancy and structure of N-linked oligosaccharides (2000), Glycobiology, 10, 251-261.
    View publication on PubMed
Search for more literature for 3.2.1.49

Cloned(Commentary)

Cloned (Comment) Organism
expression in Chines hamster ovary cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
N124Q 35% activity in comparison to 100% activity of wild-type enzyme Homo sapiens
N177Q 48% activity in comparison to 100% activity of wild-type enzyme Homo sapiens
N201Q 28% activity in comparison to 100% activity of wild-type enzyme Homo sapiens
N359Q 57% activity in comparison to 100% activity of wild-type enzyme Homo sapiens
N385Q 4% activity in comparison to 100% activity of wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is highly selective for terminal N-acetylgalactosamine residues Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More study of oligosaccharide structure, the first five: N124, N177, N201, N359 and N385, of six potential N-glycosylation sites are occupied Homo sapiens

Synonyms

Synonyms Comment Organism
alpha-galactosidase B
-
 Homo sapiens
alpha-GalNAc
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 comparison of stability at 42°C of mutant and wild-type enzymes Homo sapiens