Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gallic acid | inhibition of brush border sucrase by polyphenols in mouse intestine. Inhibition by gallic acid is a pure V effect at pH 5.0, which changes to mixed type at pH 7.2, and pure K effect at pH 8.5 | Mus musculus | |
additional information | inhibition of mice sucrase by polyphenols is pH-dependent, and is associated with conformational modifications of the enzyme. At pH 4.8, the polyphenols inhibit sucrase activity by 85-96%, which is reduced to 51 and 64%, respectively, at pH 7.2. However, at pH 8.5, 60 and 76% inhibition of enzyme activity | Mus musculus | |
Tannic acid | inhibition of brush border sucrase by polyphenols in mouse intestine. Sucrase inhibition by tannic acid is a pure K effect at acidic pH and uncompetitive type in the alkaline pH range | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values in presence of inhibitors, at pH 7.2, both gallic acid and tannic acid show mixed-type enzyme inhibition, Km is enhanced by 77-114% and Vmax by 55-60%, overview | Mus musculus | |
96.7 | - |
sucrose | pH 8.5 | Mus musculus | |
130 | - |
sucrose | pH 5.0 | Mus musculus | |
138 | - |
sucrose | pH 7.2 | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
brush border | - |
Mus musculus | 5903 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
210000 | - |
x * 210000, about | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | Mus musculus | - |
alpha-D-glucose + D-fructose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from brush border 155fold by isolation from the microvillus membranes by papain, gel filtration and anion exchange chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Mus musculus | alpha-D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 210000, about | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Mus musculus |