Literature summary for 3.2.1.48 extracted from

Champion, E.; Remaud-Simeon, M.; Skov, L.K.; Kastrup, J.S.; Gajhede, M.; Mirza, O.
The apo structure of sucrose hydrolase from Xanthomonas campestris pv. campestris shows an open active-site groove (2009), Acta Crystallogr. Sect. D, 65, 1309-1314.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme in the resting state with an empty active site, X-ray diffraction structure determination and analysis at 1.9 A resolution	Xanthomonas campestris pv. campestris

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0607	-	sucrose	-	Xanthomonas campestris pv. campestris

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + H2O	Xanthomonas campestris pv. campestris	-	alpha-D-glucose + D-fructose	-	?

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas campestris pv. campestris	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + H2O	-	Xanthomonas campestris pv. campestris	alpha-D-glucose + D-fructose	-	?
sucrose + H2O	substrate binding site and structure, overview	Xanthomonas campestris pv. campestris	alpha-D-glucose + D-fructose	-	?

Subunits

Subunits	Comment	Organism
More	residues Arg516 and Asp138 are not engaged in salt-bridge formation in the resting enzyme from Xanthomonas campestris pv. campestris in contrast to the enzyme from Xanthomonas axonopodis pv. glycines. In the absence of the salt bridge an opening is created which gives access to subsite 1 from the nonreducing end	Xanthomonas campestris pv. campestris

Synonyms

Synonyms	Comment	Organism
More	the enzyme belongs to the subfamily 4 of the glycoside hydrolase family 13, GH-13	Xanthomonas campestris pv. campestris
sucrose hydrolase	-	Xanthomonas campestris pv. campestris

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
21.7	-	sucrose	-	Xanthomonas campestris pv. campestris

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.8	8.6	-	Xanthomonas campestris pv. campestris

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Release 2023.1 (January 2023)