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Literature summary for 3.2.1.48 extracted from

  • Norman, J.M.; Bunny, K.L.; Giffard, P.M.
    Characterization of levJ, a sucrase/fructanase-encoding gene from Actinomyces naeslundii T14V, and comparison of its product with other sucrose-cleaving enzymes (1995), Gene, 152, 93-98.
    View publication on PubMed
Search for more literature for 3.2.1.48

Cloned(Commentary)

Cloned (Comment) Organism
gene levJ from a library of T14V DNA constructed in plasmid pUC18, pPNG102 contains the ORF for the sucrase polypeptide Actinomyces naeslundii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99320
-
 gene coding sucrase cloned in pPNG102 contains a ORF which defines a polypeptide of 99319 Da, the sequence shows similarity to previously sequenced sucrose-hydrolyzing enzymes Actinomyces naeslundii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltose + H2O Actinomyces naeslundii
-
 2 glucose
-
 ?
maltose + H2O Actinomyces naeslundii T14V
-
 2 glucose
-
 ?
sucrose + H2O Actinomyces naeslundii
-
 D-glucose + D-fructose
-
 ?
sucrose + H2O Actinomyces naeslundii T14V
-
 D-glucose + D-fructose
-
 ?

Organism

Organism UniProt Comment Textmining
Actinomyces naeslundii
-
 T14V
-
Actinomyces naeslundii T14V
-
 T14V
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
 Actinomyces naeslundii ?
-
 ?
inulin + H2O
-
 Actinomyces naeslundii T14V ?
-
 ?
levan + H2O
-
 Actinomyces naeslundii ?
-
 ?
levan + H2O
-
 Actinomyces naeslundii T14V ?
-
 ?
maltose + H2O
-
 Actinomyces naeslundii 2 glucose
-
 ?
maltose + H2O
-
 Actinomyces naeslundii T14V 2 glucose
-
 ?
raffinose + H2O
-
 Actinomyces naeslundii ?
-
 ?
raffinose + H2O
-
 Actinomyces naeslundii T14V ?
-
 ?
sucrose + H2O
-
 Actinomyces naeslundii D-glucose + D-fructose
-
 ?
sucrose + H2O
-
 Actinomyces naeslundii T14V D-glucose + D-fructose
-
 ?