Literature summary for 3.2.1.48 extracted from

Quezada-Calvillo, R.; Markowitz, A.J.; Traber, P.G.; Underdown, B.J.
Murine intestinal disaccharidases: identification of structural variants of sucrase-isomaltose complex (1993), Am. J. Physiol., 265, 1142-1149.

No PubMed abstract available

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Cloned(Commentary)

Cloned (Comment)	Organism
mouse SI mRNA synthesized from mouse SI cDNA, pMSI-A1 cloned into pBluescript KS, cDNA cloned from mouse intestinal RNA with anchored polymerase chain reaction method	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
20.2	21.2	sucrose	inbred strains of mice with genetic and posttranslational variations in SI complex structure, incubation at 37°C	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
220000	-	-	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
maltose + H2O	Mus musculus	-	2 glucose	-	?
sucrose + H2O	Mus musculus	-	D-glucose + D-fructose	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	mouse, inbred strains CBA/J, CBA/Ca, CBA/CaH, CBA/CaHN, C57B1/6	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
17.5	-	-	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltose + H2O	-	Mus musculus	2 glucose	-	?
sucrose + H2O	-	Mus musculus	D-glucose + D-fructose	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	pH 5.9, inactivation, soluble enzyme activity is stable to heat treatment, membrane associated enzyme was heat-labile under these conditions	Mus musculus

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Release 2023.1 (January 2023)