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Literature summary for 3.2.1.48 extracted from
- Separation and characterization of sucrose-isomaltase and of glucoamylase of rat intestine (1972), Biochim. Biophys. Acta, 284, 235-247.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-Bromo-2-naphthyl-alpha-glucoside | - |
Rattus rattus |  |
| maltose | - |
Rattus rattus | |
| p-Nitrophenyl-alpha-glucoside | competitive inhibitor, inhibits both sucrase and isomaltase | Rattus rattus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
6-bromo-2-naphthyl-alpha-D-glucoside | - |
Rattus rattus | |
| 1.5 | - |
p-nitrophenyl-alpha-D-glucoside | - |
Rattus rattus | |
| 3.1 | - |
p-nitrophenyl-alpha-D-glucoside | - |
Rattus rattus | |
| 7.2 | - |
maltose | - |
Rattus rattus | |
| 11.5 | - |
isomaltose | - |
Rattus rattus | |
| 13.5 | - |
maltose | the existence of 2 Km values supports the notion of substrate hydrolysis at 2 active sites on the sucrase-isomaltase complex | Rattus rattus | |
| 30.8 | - |
sucrose | - |
Rattus rattus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | - |
Rattus rattus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltose + H2O | Rattus rattus | - |
glucose | - |
? | |
| maltose + H2O | Rattus rattus | - |
2 glucose | - |
? | |
| sucrose + H2O | Rattus rattus | - |
D-glucose + D-fructose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus rattus | - |
sucrase-isomaltase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus rattus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-bromo-2-naphthyl-alpha-D-glucoside + H2O | - |
Rattus rattus | 6-bromonaphthol + alpha-D-glucose | - |
? | |
| dextran + H2O | - |
Rattus rattus | D-glucose | - |
? | |
| isomaltose + H2O | - |
Rattus rattus | glucose | - |
? | |
| isomaltose + H2O | - |
Rattus rattus | 2 D-glucose | - |
? | |
| maltose + H2O | - |
Rattus rattus | 2 glucose | - |
? | |
| maltose + H2O | - |
Rattus rattus | 2 D-glucose | - |
? | |
| p-nitrophenyl-alpha-glucoside + H2O | - |
Rattus rattus | p-nitrophenol + D-glucose | - |
? | |
| sucrose + H2O | - |
Rattus rattus | D-glucose + D-fructose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 5.9, inactivation, soluble enzyme activity is stable to heat treatment, membrane associated enzyme was heat-labile under these conditions | Rattus rattus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
in the presence of 50 mM Na+ | Rattus rattus |
| 6.7 | - |
- |
Rattus rattus |
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