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Literature summary for 3.2.1.45 extracted from

  • Massimo, A.; Maura, S.; Nicoletta, L.; Giulia, M.; Valentina, M.; Elena, C.; Alessandro, P.; Rosaria, B.; Sandro, S.
    Current and novel aspects on the non-lysosomal beta-glucosylceramidase GBA2 (2016), Neurochem. Res., 41, 210-220 .
    View publication on PubMed
Search for more literature for 3.2.1.45

Activating Compound

Activating Compound Comment Organism Structure
acetazolamide
-
 Homo sapiens
EGTA
-
 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in COS-7 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-(N-ethyl-N-isopropyl)amiloride
-
 Homo sapiens
conduritol B epoxide isoform GBA2 is inactivated by conduritol B epoxide in a time-dependent manner. When 0.5 mM conduritol B epoxide is administered to cultured cells for 2 days, isoform GBA2 activity is reduced about 50-60% Homo sapiens
deoxynojirimycin
-
 Homo sapiens
esomeprazole
-
 Homo sapiens
N-(5-adamantane-1-yl-methoxypentyl)deoxynojirimycin most powerful inhibitor of isoform GBA2 Homo sapiens
N-butyl deoxynojirimycin miglustat or Zavesca Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
Golgi apparatus
-
 Homo sapiens 5794
-
plasma membrane cytosolic side of membranes Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucocerebroside + H2O Homo sapiens
-
 D-glucose + ceramide
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HCG7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cerebrum
-
 Homo sapiens
-
colon
-
 Homo sapiens
-
forebrain highest activity Homo sapiens
-
glioma cell
-
 Homo sapiens
-
heart
-
 Homo sapiens
-
hepatoma cell
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
leukocyte
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
lymphocyte
-
 Homo sapiens
-
neuroblastoma cell
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-
skeletal muscle
-
 Homo sapiens
-
skin fibroblast
-
 Homo sapiens
-
small intestine
-
 Homo sapiens
-
spleen
-
 Homo sapiens
-
thymus
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-glucopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + beta-D-glucopyranose
-
 ?
glucocerebroside + H2O
-
 Homo sapiens D-glucose + ceramide
-
 ?

Subunits

Subunits Comment Organism
? x * 104000, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
beta-glucosidase 2
-
 Homo sapiens
GBA2
-
 Homo sapiens
microsomal bile acid beta-glucosidase
-
 Homo sapiens
non-lysosomal beta-glucosylceramidase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 the enzyme shows more than 50% activity between pH 5.0 and 7.5 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
8.1
-
 conduritol B epoxide pH and temperature not specified in the publication Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens calculated from amino acid sequence
-
 5.6