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Literature summary for 3.2.1.45 extracted from

  • Reddy, P.U.M.; Murray, G.J.; Barranger, J.A.
    Purification and characterization of bovine brain glucocerebrosidase (1985), Biochem. Med., 33, 200-210.
    View publication on PubMed
Search for more literature for 3.2.1.45

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
(1R,2R,3S,4S,5R,6S)-7-oxabicyclo[4.1.0]heptane-2,3,4,5-tetrol
-
 Bos taurus
gluconolactone competitive inhibitor Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 beta-D-glucocerebroside
-
 Bos taurus
3
-
 4-methylumbelliferyl-beta-D-glucopyranoside
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 gel filtration, SDS-PAGE Bos taurus
63000
-
 SDS-PAGE Bos taurus
138000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucocerebroside + H2O Bos taurus
-
 glucose + ceramide
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Bos taurus methylumbelliferone + glucose
-
 ?
glucocerebroside + H2O
-
 Bos taurus glucose + ceramide
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 60 min, pH 5.8, 70% loss of activity Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5
-
 Bos taurus