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Literature summary for 3.2.1.41 extracted from

  • Liu, J.; Liu, Y.; Yan, F.; Jiang, Z.; Yang, S.; Yan, Q.
    Gene cloning, functional expression and characterisation of a novel type I pullulanase from Paenibacillus barengoltzii and its application in resistant starch production (2016), Protein Expr. Purif., 121, 22-30 .
    View publication on PubMed
Search for more literature for 3.2.1.41

Application

Application Comment Organism
food industry useful in starch industries. The addition of the enzyme in gelatinized rice and maize starches significantly increases the resistant starch type 3 yields (RS3). RS3 is of great importance for nutritionists and food industry, due to its reduced levels of plasma glucose and insulin, increased faecal bulk, and short-chain fatty acid production through fermentation in the large intestine Paenibacillus barengoltzii

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli as an intracellular soluble protein Paenibacillus barengoltzii

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 84% loss of activity Paenibacillus barengoltzii
Cu2+ 1 mM, 82% loss of activity Paenibacillus barengoltzii
Hg2+ 1 mM, 99% loss of activity Paenibacillus barengoltzii
Sn2+ 1 mM, 87% loss of activity Paenibacillus barengoltzii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 1.6fold activation Paenibacillus barengoltzii
Cr2+ 1 mM, 1.1fold activation Paenibacillus barengoltzii
Fe3+ 1 mM, 1.4fold activation Paenibacillus barengoltzii
K+ 1 mM, 1.2fold activation Paenibacillus barengoltzii
Mg2+ 1 mM, 1.1fold activation Paenibacillus barengoltzii
Ni2+ 1 mM, 1.1fold activation Paenibacillus barengoltzii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 SDS-PAGE Paenibacillus barengoltzii
75093
-
 calculated from sequence Paenibacillus barengoltzii
78000
-
 gel filtration Paenibacillus barengoltzii

Organism

Organism UniProt Comment Textmining
Paenibacillus barengoltzii A0A0C5GWS2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Paenibacillus barengoltzii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 12% of the activity compared to pullulan Paenibacillus barengoltzii ?
-
 ?
pullulan + H2O the enzyme exhibits strict substrate specificity towards pullulan, but shows relatively low activity towards amylopectin and no activity towards other tested polysaccharides Paenibacillus barengoltzii maltotriose + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Paenibacillus barengoltzii

Synonyms

Synonyms Comment Organism
PbPulA
-
 Paenibacillus barengoltzii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenibacillus barengoltzii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55 40°C: about 45% of maximal activity, 55°C: about 30% of maximal activity Paenibacillus barengoltzii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 thermal denaturing half-lives: 501 min Paenibacillus barengoltzii
45
-
 thermal denaturing half-lives: 253 min Paenibacillus barengoltzii
50
-
 thermal denaturing half-lives: 33 min Paenibacillus barengoltzii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 pullulan Km-value: 2.94 mg/ml, pH 5.5, 50°C Paenibacillus barengoltzii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Paenibacillus barengoltzii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10.5 30 min, enzyme retains more than 80% of its activity Paenibacillus barengoltzii

pI Value

Organism Comment pI Value Maximum pI Value
Paenibacillus barengoltzii calculated from sequence
-
 5.22