Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.41 extracted from

  • Chen, A.; Xu, T.; Ge, Y.; Wang, L.; Tang, W.; Li, S.
    Hydrogen-bond-based protein engineering for the acidic adaptation of Bacillus acidopullulyticus pullulanase (2019), Enzyme Microb. Technol., 124, 79-83 .
    View publication on PubMed
Search for more literature for 3.2.1.41

Application

Application Comment Organism
food industry break down of starch for the production of high-glucose syrup. The L627R mutant enzyme may be suitable for industrial application because its shortened reaction time translates to reduced energy consumption Bacillus acidopullulyticus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Bacillus acidopullulyticus

Protein Variants

Protein Variants Comment Organism
L627R the pH optimum of the mutant enzyme shifts from 5.0 to 4.0, and its relative activity at pH 4.0 is 117% that of the wide-type enzyme. The L627R mutant exhibits increased tolerance against acid-mediated denaturation, and its maximum D-glucose content (97.4%) is obtained after 40 h incubation, which is shorter by 10 h compared with the time required by the wild-type enzyme to produce a comparable amount of the monosaccharide. The L627R mutant may be suitable for industrial application because its shortened reaction time translates to reduced energy consumption Bacillus acidopullulyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 pullulan Km-value: 0.61 mg/ml, pH 5.0, 60°C, mutant enzyme L627R Bacillus acidopullulyticus
additional information
-
 pullulan Km-value: 0.93 mg/ml, pH 5.0, 60°C, wild-type enzyme Bacillus acidopullulyticus

Organism

Organism UniProt Comment Textmining
Bacillus acidopullulyticus Ax203843.1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus acidopullulyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
984.2
-
 substrate: pullulan, pH 5.0, 60°C, wild-type enzyme Bacillus acidopullulyticus
1178.5
-
 substrate: pullulan, pH 5.0, 60°C, mutant enzyme L627R Bacillus acidopullulyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pullulan + H2O
-
 Bacillus acidopullulyticus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Bacillus acidopullulyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
770.4
-
 pullulan pH 5.0, 60°C, mutant enzyme L627R Bacillus acidopullulyticus
977.3
-
 pullulan pH 5.0, 60°C, wild-type enzyme Bacillus acidopullulyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bacillus acidopullulyticus