Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
pullulan | Km-value: 3.3 mg/ml, pH 6.5, 55°C | Priestia megaterium | |
additional information | - |
amylopectin | Km-value: 3.6 mg/ml, pH 6.5, 55°C | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106063 | - |
calculated from sequence | Priestia megaterium |
112000 | - |
SDS-PAGE | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | A0A173DUJ5 | - |
- |
Priestia megaterium WW1210 | A0A173DUJ5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | 28% of the activity compared to pullulan, the enzyme hydrolyzes amylopectin to release mainly maltotriose, maltotetraose, maltopentaose and maltohexaose, and their contents reach to 0.0044, 0.0068, 0.0073, 0.025 and 0.035 mg/ml, respectively, after 2 h incubation | Priestia megaterium | maltotriose + maltotetraose + maltopentaose + maltohexaose | - |
? | |
amylopectin + H2O | 28% of the activity compared to pullulan, the enzyme hydrolyzes amylopectin to release mainly maltotriose, maltotetraose, maltopentaose and maltohexaose, and their contents reach to 0.0044, 0.0068, 0.0073, 0.025 and 0.035 mg/ml, respectively, after 2 h incubation | Priestia megaterium WW1210 | maltotriose + maltotetraose + maltopentaose + maltohexaose | - |
? | |
additional information | the enzyme does not show activity towards amylose, maltoheptaose, maltohexaose, maltopentaose and maltotetraose | Priestia megaterium | ? | - |
? | |
additional information | the enzyme does not show activity towards amylose, maltoheptaose, maltohexaose, maltopentaose and maltotetraose | Priestia megaterium WW1210 | ? | - |
? | |
pullulan + H2O | the enzyme hydrolyzes pullulan to yield mainly maltotriose, more than 95% | Priestia megaterium | maltotriose + ? | - |
? | |
pullulan + H2O | the enzyme hydrolyzes pullulan to yield mainly maltotriose, more than 95% | Priestia megaterium WW1210 | maltotriose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 112000, SDS-PAGE | Priestia megaterium |
? | x * 106063, calculated from sequence | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
BmPul | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Priestia megaterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | 40°C: about 35% of maximal activity, 60°C: about 25% of maximal activity | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable below | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Priestia megaterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 45% of maximal activity, pH 9.0: about 25% of maximal activity | Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | 30 min, enzyme retains more than 85% of its original activity | Priestia megaterium |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Priestia megaterium | calculated from sequence | - |
7.7 |