General Stability | Organism |
---|---|
the pullulanases immobilized on Florisil and nano-silica protected 71% and 90% of their initial activities after 10 reuses | Fontibacillus sp. DSHK 107 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
pullulan | pH 5.0, 30°C, the apparent kcat/Km-values are 1.49, 1.54, and 0.59 mg/ml pullunan, respectively, for the soluble enzyme and pullulanase immobilized on Florisil and nano-silica | Fontibacillus sp. DSHK 107 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fontibacillus sp. DSHK 107 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Fontibacillus sp. DSHK 107 |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pullulan + H2O | - |
Fontibacillus sp. DSHK 107 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
soluble enzyme and immobilized pullulanases on Florisil and nano-silica through glutaraldehyde spacer arm | Fontibacillus sp. DSHK 107 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
t1/2 of soluble enzyme: 8.9 min. Thermal stability of pullulanase immobilized on Florisil and nano-silica is enhanced 6.5- and 15.6fold | Fontibacillus sp. DSHK 107 |
50 | - |
t1/2 of soluble enzyme: 6.2 min. Thermal stability of pullulanase immobilized on Florisil and nano-silica is enhanced 6.6- and 16.0fold | Fontibacillus sp. DSHK 107 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
immobilized pullulanases on Florisil and nano-silica through glutaraldehyde spacer arm | Fontibacillus sp. DSHK 107 |
6 | - |
soluble enzyme | Fontibacillus sp. DSHK 107 |
General Information | Comment | Organism |
---|---|---|
additional information | pullulanase partially purified from Fontibacillus sp. is covalently immobilized on Florisil and nano-silica through both glutaraldehyde and (3-glycidyloxypropyl)trimethoxysilane spacer arms. The pullulanase immobilized on Florisil and nano-silica through glutaraldehyde spacer arm show 85% and 190% activity of its free form, respectively, whereas no activity is observed when it is immobilized on the same supports through (3-glycidyloxypropyl)trimethoxysilane spacer arm | Fontibacillus sp. DSHK 107 |