Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, enhances activity 1.4fold | Thermus thermophilus |
Application | Comment | Organism |
---|---|---|
industry | the enzyme can be employed for industrial saccharification of starch | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli using pHsh and pET28a vectors. The pHsh expression system produces a 3.6fold higher recombinant pullulanase than pET28a | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cr3+ | 5 mM, 34% inhibition, pH 6.5, 70°C | Thermus thermophilus | |
Cu2+ | 5 mM, complete inhibition, pH 6.5, 70°C | Thermus thermophilus | |
Hg2+ | 5 mM, complete inhibition, pH 6.5, 70°C | Thermus thermophilus | |
Ni2+ | 5 mM, 31% inhibition, pH 6.5, 70°C | Thermus thermophilus | |
Pb2+ | 5 mM, 75% inhibition, pH 6.5, 70°C | Thermus thermophilus | |
SDS | 10 mM, 96% inhibition, pH 6.5, 70°C | Thermus thermophilus | |
Zn2+ | 5 mM, 58% inhibition, pH 6.5, 70°C | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
pullulan | Km-value: 0.0031 mg/ml | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 5 mM, enhances activity 1.7fold, pH 6.5, 70°C | Thermus thermophilus | |
Mg2+ | 5 mM, enhances activity 1.2fold, pH 6.5, 70°C | Thermus thermophilus | |
Mn2+ | 5 mM, enhances activity up to 3fold, pH 6.5, 70°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
SDS-PAGE | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72ID4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pullulan + H2O | this enzyme can attack alpha-1,6- and alpha-1,4-glycosidic linkages in pullulan, and produce a mixture of maltotriose, maltose and glucose | Thermus thermophilus | maltotriose + maltose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TTC1198 | - |
Thermus thermophilus |
TTHpu | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
His6-tagged recombinant enzyme, at pH 6.5 | Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 95 | 45°C: about 60% of maximal activity, 95°C: about 55% of maximal activity | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
1 h, residual activity is more than 90% | Thermus thermophilus |
70 | - |
1 h, residual activity is more than 90%. More than 70% of the activity is retained after 1 h at pH 6-8 with the absence of the substrate | Thermus thermophilus |
80 | - |
half-life: 2 h | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5.0: about 65% of maximal activity, pH 8.5: about 65% of maximal activity | Thermus thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 1 h, 70°C, stable | Thermus thermophilus |