Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, 131% of initial activity | Thermotoga neapolitana | |
dithiothreitol | 1 mM, 168% of initial activity | Thermotoga neapolitana |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga neapolitana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 18% residual activity | Thermotoga neapolitana | |
Cu2+ | 1 mM, complete inactivation | Thermotoga neapolitana | |
Hg2+ | 1 mM, complete inactivation | Thermotoga neapolitana | |
Mn2+ | 1 mM, 15% residual activity | Thermotoga neapolitana | |
additional information | not inhibitory: EDTA | Thermotoga neapolitana | |
N-bromosuccinimide | complete inactivation | Thermotoga neapolitana | |
Ni2+ | 1 mM, 18% residual activity | Thermotoga neapolitana | |
Zn2+ | 1 mM, 53% residual activity | Thermotoga neapolitana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93000 | - |
x * 93000, SDS-PAGE | Thermotoga neapolitana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | C0LLG9 | type I enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a 19-residue signal peptide | Thermotoga neapolitana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.7 | - |
pH 6.5, 80°C | Thermotoga neapolitana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
glycogen + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
additional information | no substrate: amylose. Enzyme displays transglycosylation activity, transferring the maltotriosyl residue of pullulan to aesculin by forming alpha-1,6-glucosidic linkages | Thermotoga neapolitana | ? | - |
? | |
pullulan + H2O | - |
Thermotoga neapolitana | maltotriose + ? | - |
? | |
starch + H2O | - |
Thermotoga neapolitana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 93000, SDS-PAGE | Thermotoga neapolitana |
Synonyms | Comment | Organism |
---|---|---|
PulA | - |
Thermotoga neapolitana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermotoga neapolitana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life 88 min | Thermotoga neapolitana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Thermotoga neapolitana |