Any feedback?
Literature summary for 3.2.1.41 extracted from
- Heterologous expression, secretion and characterization of the Geobacillus thermoleovorans US105 type I pullulanase (2008), Appl. Microbiol. Biotechnol., 78, 473-481.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, connection to the lipase A signal peptide of Bacillus subtilis strain leads to an efficient secretion of an active form enzyme on the periplasmic fraction, fusion to the amylase signal sequence of the Bacillus stearothermophilus US100 strain leads to an efficient secretion of this natively cytoplasmic enzyme as an active form in the supernatant | Geobacillus thermoleovorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyclodextrin | - |
Geobacillus thermoleovorans | |
| additional information | EDTA has no effect | Geobacillus thermoleovorans | |
| SDS | - |
Geobacillus thermoleovorans |  |
| Triton X-100 | - |
Geobacillus thermoleovorans | |
| Urea | - |
Geobacillus thermoleovorans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stabilizes the enzyme at temperatures above 45°C | Geobacillus thermoleovorans | |
| Co2+ | inhibitory | Geobacillus thermoleovorans | |
| Cu2+ | complete inhibitory | Geobacillus thermoleovorans | |
| Fe2+ | inhibitory | Geobacillus thermoleovorans | |
| Mn2+ | complete inhibitory | Geobacillus thermoleovorans | |
| additional information | Mg2+, Zn2+ have no activity | Geobacillus thermoleovorans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
2 * 80000 | Geobacillus thermoleovorans |
| 160000 | - |
SDS-PAGE | Geobacillus thermoleovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermoleovorans | - |
previously B. thermoleovorans US105 | - |
| Geobacillus thermoleovorans US105 | - |
previously B. thermoleovorans US105 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein using heat treatment, size exclusion, and anion-exchange chromatography | Geobacillus thermoleovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.2 | - |
of the periplasmic homogenate before purification | Geobacillus thermoleovorans |
| 12 | - |
in the supernatant | Geobacillus thermoleovorans |
| 36 | - |
of the periplasmic homogenate after purification | Geobacillus thermoleovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pullulan + H2O | - |
Geobacillus thermoleovorans | maltotriose + maltose | - |
? | |
| pullulan + H2O | - |
Geobacillus thermoleovorans US105 | maltotriose + maltose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 80000 | Geobacillus thermoleovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PUL US105 | - |
Geobacillus thermoleovorans |
| pullulanase | - |
Geobacillus thermoleovorans |
| pullulanase type 1 | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
with 1 mM CaCl2 | Geobacillus thermoleovorans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 70 | - |
Geobacillus thermoleovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
with 1 mM CaCl2 | Geobacillus thermoleovorans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
