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Literature summary for 3.2.1.41 extracted from
- Enzymatic properties and regulation of ZPU1, the maize pullulanase-type starch debranching enzyme (2002), Arch. Biochem. Biophys., 406, 21-32.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | upon preincubation with both dithiothreitol and thioredoxin, the initial reaction velocity increases by a factor of two | Zea mays |  |
| thioredoxin | upon preincubation with both dithiothreitol and thioredoxin, the initial reaction velocity increases by a factor of two | Zea mays | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
pullulan | pH 6.0, 37°C | Zea mays | |
| 4.1 | - |
beta-limit dextrin | pH 6.0, 37°C | Zea mays | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | O81638 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein expressed in Escherichia coli | Zea mays |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.5 | - |
substrate phytoglycogen, pH 6.0, 37°C | Zea mays |
| 32.7 | - |
substrate amylopectin, pH 6.0, 37°C | Zea mays |
| 165.9 | - |
substrate pullulan, pH 6.0, 37°C | Zea mays |
| 223.9 | - |
substrate beta-limit dextrin, pH 6.0, 37°C | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | 20% of activity compared to pullulan | Zea mays | ? | - |
? | |
| beta-limit dextrin + H2O | - |
Zea mays | ? | - |
? | |
| beta-limit dextrin + H2O | 135% of activity compared to pullulan | Zea mays | ? | - |
? | |
| additional information | enzyme requires at least two glucose units in linear chain to be released by cleavage of an alpha-1,6-linkage | Zea mays | ? | - |
? | |
| additional information | no substrate: phytogen | Zea mays | ? | - |
? | |
| phytoglycogen + H2O | 9% of activity compared to pullulan | Zea mays | ? | - |
? | |
| pullulan + H2O | - |
Zea mays | ? | - |
? | |
| pullulan + H2O | - |
Zea mays | maltotriose + ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
stable | Zea mays |
| 50 | - |
complete inactivation within 15 min | Zea mays |
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