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Literature summary for 3.2.1.41 extracted from

  • Bertoldo, C.; Duffner, F.; Jorgensen, P.L.; Antranikian, G.
    Pullulanase type I from Fervidobacterium pennavorans Ven5: cloning, sequencing, and expression of the gene and biochemical characterization of the recombinant enzyme (1999), Appl. Environ. Microbiol., 65, 2084-2091.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.41

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Fervidobacterium pennivorans
dithiothreitol
-
 Fervidobacterium pennivorans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Fervidobacterium pennivorans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ at 5 mM Fervidobacterium pennivorans
Cyclodextrins
-
 Fervidobacterium pennivorans
Fe2+ at 5 mM Fervidobacterium pennivorans
Guanidine HCl
-
 Fervidobacterium pennivorans
iodoacetamide
-
 Fervidobacterium pennivorans
N-bromosuccinimide
-
 Fervidobacterium pennivorans
Ni2+ at 5 mM Fervidobacterium pennivorans
Sodium dodecyl sulfate
-
 Fervidobacterium pennivorans
Urea
-
 Fervidobacterium pennivorans
Zn2+ at 5 mM Fervidobacterium pennivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylopectin + H2O Fervidobacterium pennivorans branched polysaccharides maltooligosaccharides + maltose
-
 ?
amylopectin + H2O Fervidobacterium pennivorans Ven5 branched polysaccharides maltooligosaccharides + maltose
-
 ?
pullulan + H2O Fervidobacterium pennivorans
-
 maltotriose + ?
-
 ?
pullulan + H2O Fervidobacterium pennivorans Ven5
-
 maltotriose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fervidobacterium pennivorans
-
 Ven5
-
Fervidobacterium pennivorans Ven5
-
 Ven5
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein Fervidobacterium pennivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
amylopectin + H2O branched polysaccharides Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
amylopectin + H2O
-
 Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose
-
 ?
amylopectin + H2O branched polysaccharides Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose
-
 ?
dextrin + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
glycogen + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
glycogen + H2O
-
 Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose
-
 ?
pullulan + H2O
-
 Fervidobacterium pennivorans maltotriose + ?
-
 ?
pullulan + H2O
-
 Fervidobacterium pennivorans Ven5 maltotriose + ?
-
 ?
soluble starch
-
 Fervidobacterium pennivorans maltotriose + maltose
-
 ?
soluble starch
-
 Fervidobacterium pennivorans Ven5 maltotriose + maltose
-
 ?

Synonyms

Synonyms Comment Organism
pullulanase type I hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan Fervidobacterium pennivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Fervidobacterium pennivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 pH 6, after 2 h, 50% loss of activity Fervidobacterium pennivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Fervidobacterium pennivorans