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Literature summary for 3.2.1.41 extracted from

  • Kim, C.H.; Choi, H.I.; Lee, D.S.
    Purification and biochemical properties of an alkaline pullulanase from alkalophilic Bacillus sp. S-1 (1993), Biosci. Biotechnol. Biochem., 57, 1632-1637.
    View publication on PubMed
Search for more literature for 3.2.1.41

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Bacillus sp. (in: Bacteria)
Fe2+
-
 Bacillus sp. (in: Bacteria)
Fe3+
-
 Bacillus sp. (in: Bacteria)
Zn2+
-
 Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Bacillus sp. (in: Bacteria)
Co2+ slightly stimulated Bacillus sp. (in: Bacteria)
K+ slightly stimulated Bacillus sp. (in: Bacteria)
Mg2+ slightly stimulated Bacillus sp. (in: Bacteria)
Mn2+ highly stimulated Bacillus sp. (in: Bacteria)
Na+ slightly stimulated Bacillus sp. (in: Bacteria)
Ni2+ slightly stimulated Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylopectin + H2O Bacillus sp. (in: Bacteria) branched polysaccharides maltooligosaccharides + maltose
-
 ?
pullulan + H2O Bacillus sp. (in: Bacteria)
-
 maltotriose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose
-
 ?
amylopectin + H2O branched polysaccharides Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose
-
 ?
dextrin + H2O
-
 Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose
-
 ?
glycogen + H2O
-
 Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose
-
 ?
glycogen beta-limit dextrin + H2O
-
 Bacillus sp. (in: Bacteria) maltotriose + maltose + maltosetetraose
-
 ?
pullulan + H2O
-
 Bacillus sp. (in: Bacteria) maltotriose + ?
-
 ?

Synonyms

Synonyms Comment Organism
pullulanase type I hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 stability increased by pullulan Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 stable at 4°C for 24 h Bacillus sp. (in: Bacteria)