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Literature summary for 3.2.1.41 extracted from

  • Saha, B.C.; Mathupala, S.P.; Zeikus, J.G.
    Purification and characterization of a highly thermostable novel pullulanase from Clostridium thermohydrosulfuricum (1988), Biochem. J., 252, 343-348.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Thermoanaerobacter thermohydrosulfuricus

Inhibitors

Inhibitors Comment Organism Structure
Cyclodextrins
-
Thermoanaerobacter thermohydrosulfuricus
EDTA
-
Thermoanaerobacter thermohydrosulfuricus
Fe3+
-
Thermoanaerobacter thermohydrosulfuricus
N-bromosuccinimide
-
Thermoanaerobacter thermohydrosulfuricus
Zn2+
-
Thermoanaerobacter thermohydrosulfuricus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Thermoanaerobacter thermohydrosulfuricus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Thermoanaerobacter thermohydrosulfuricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
133000
-
gel filtration Thermoanaerobacter thermohydrosulfuricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylopectin + H2O Thermoanaerobacter thermohydrosulfuricus branched polysaccharides maltooligosaccharides + maltose
-
?
pullulan + H2O Thermoanaerobacter thermohydrosulfuricus
-
maltotriose + ?
-
?

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter thermohydrosulfuricus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Thermoanaerobacter thermohydrosulfuricus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Thermoanaerobacter thermohydrosulfuricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
481
-
-
Thermoanaerobacter thermohydrosulfuricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose
-
?
amylopectin + H2O branched polysaccharides Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose
-
?
glycogen + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose
-
?
glycogen beta-limit dextrin + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltotriose + maltose + maltosetetraose
-
?
pullulan + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltotriose + ?
-
?
soluble starch
-
Thermoanaerobacter thermohydrosulfuricus maltotriose + maltose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 133000, gel electrophoresis Thermoanaerobacter thermohydrosulfuricus

Synonyms

Synonyms Comment Organism
pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Thermoanaerobacter thermohydrosulfuricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Thermoanaerobacter thermohydrosulfuricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
instable above Thermoanaerobacter thermohydrosulfuricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
271
-
pullulan
-
Thermoanaerobacter thermohydrosulfuricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Thermoanaerobacter thermohydrosulfuricus

pH Range

pH Minimum pH Maximum Comment Organism
8
-
not active above Thermoanaerobacter thermohydrosulfuricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5
-
Thermoanaerobacter thermohydrosulfuricus