Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Thermoanaerobacter thermohydrosulfuricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cyclodextrins | - |
Thermoanaerobacter thermohydrosulfuricus | |
EDTA | - |
Thermoanaerobacter thermohydrosulfuricus | |
Fe3+ | - |
Thermoanaerobacter thermohydrosulfuricus | |
N-bromosuccinimide | - |
Thermoanaerobacter thermohydrosulfuricus | |
Zn2+ | - |
Thermoanaerobacter thermohydrosulfuricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Thermoanaerobacter thermohydrosulfuricus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Thermoanaerobacter thermohydrosulfuricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
133000 | - |
gel filtration | Thermoanaerobacter thermohydrosulfuricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | Thermoanaerobacter thermohydrosulfuricus | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
pullulan + H2O | Thermoanaerobacter thermohydrosulfuricus | - |
maltotriose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter thermohydrosulfuricus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Thermoanaerobacter thermohydrosulfuricus |
Purification (Comment) | Organism |
---|---|
to homogeneity | Thermoanaerobacter thermohydrosulfuricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
481 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltooligosaccharides + maltose | - |
? | |
amylopectin + H2O | branched polysaccharides | Thermoanaerobacter thermohydrosulfuricus | maltooligosaccharides + maltose | - |
? | |
glycogen + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltooligosaccharides + maltose | - |
? | |
glycogen beta-limit dextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltotriose + maltose + maltosetetraose | - |
? | |
pullulan + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltotriose + ? | - |
? | |
soluble starch | - |
Thermoanaerobacter thermohydrosulfuricus | maltotriose + maltose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 133000, gel electrophoresis | Thermoanaerobacter thermohydrosulfuricus |
Synonyms | Comment | Organism |
---|---|---|
pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Thermoanaerobacter thermohydrosulfuricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
instable above | Thermoanaerobacter thermohydrosulfuricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
271 | - |
pullulan | - |
Thermoanaerobacter thermohydrosulfuricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
not active above | Thermoanaerobacter thermohydrosulfuricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | - |
Thermoanaerobacter thermohydrosulfuricus |