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Literature summary for 3.2.1.41 extracted from
- Purification and properties of Bacillus acidopullulyticus pullulanase (1988), Agric. Biol. Chem., 52, 2293-2298.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
pullulan | glucose equivalents | Bacillus acidopullulyticus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
1 * 115000, gel electrophoresis | Bacillus acidopullulyticus |
| 115000 | 116000 | gel electrophoresis | Bacillus acidopullulyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Bacillus acidopullulyticus | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Bacillus acidopullulyticus | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus acidopullulyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus acidopullulyticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | promozym 200L | Bacillus acidopullulyticus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | 220 | - |
Bacillus acidopullulyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Bacillus acidopullulyticus | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Bacillus acidopullulyticus | maltooligosaccharides + maltose | - |
? | |
| glycogen beta-limit dextrin + H2O | - |
Bacillus acidopullulyticus | maltotriose + maltose + maltosetetraose | - |
? | |
| pullulan + H2O | - |
Bacillus acidopullulyticus | maltotriose + ? | - |
? | |
| soluble starch | - |
Bacillus acidopullulyticus | maltotriose + maltose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 115000, gel electrophoresis | Bacillus acidopullulyticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type I | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan | Bacillus acidopullulyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Bacillus acidopullulyticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
not active above, pullulan | Bacillus acidopullulyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
instable above | Bacillus acidopullulyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Bacillus acidopullulyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | - |
Bacillus acidopullulyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | - |
Bacillus acidopullulyticus |
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