Application | Comment | Organism |
---|---|---|
biotechnology | potential use as a biocatalyst for diverse biotechnological applications | Novosphingobium sp. PP1Y |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Novosphingobium sp. PP1Y |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160.2 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, temperature not specified in the publication | Novosphingobium sp. PP1Y |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101000 | - |
gel filtration | Novosphingobium sp. PP1Y |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 10% acetone, 50 mM MOPS, pH 6.9, 6.73% residual activity. Complete loss of activity in 50% acetone | Novosphingobium sp. PP1Y |
DMSO | 10% DMSO, in 50 mM MOPS, pH 6.9, 66.29% residual activity. Complete loss of activity in 50% DMSO | Novosphingobium sp. PP1Y |
Ethanol | 10% ethanol, 50 mM MOPS, pH 6.9, 66.52% residual activity. Complete loss of activity in 50% ethanol | Novosphingobium sp. PP1Y |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Novosphingobium sp. PP1Y | F6IEX3 | - |
- |
Purification (Comment) | Organism |
---|---|
expression in Escherichia coli, strain BL21(DE3) | Novosphingobium sp. PP1Y |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-rhamnopyranoside + H2O | - |
Novosphingobium sp. PP1Y | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
naringin + H2O | - |
Novosphingobium sp. PP1Y | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
neohesperidin dihydrochalcone + H2O | - |
Novosphingobium sp. PP1Y | 3,5-dihydroxy-4-[3-(3-hydroxy-4-methoxyphenyl)propanoyl]phenyl beta-D-glucopyranoside + alpha-L-rhamnose | - |
? | |
rutin + H2O | - |
Novosphingobium sp. PP1Y | isoquercitrin + alpha-L-rhamnose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Novosphingobium sp. PP1Y |
Synonyms | Comment | Organism |
---|---|---|
alpha-RHA | - |
Novosphingobium sp. PP1Y |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40.9 | - |
- |
Novosphingobium sp. PP1Y |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | 45 | 32°C: about 55% of maximal activity, 45°C: about 65% of maximal activity | Novosphingobium sp. PP1Y |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | 3 h, stable | Novosphingobium sp. PP1Y |
40 | - |
24 h, stable | Novosphingobium sp. PP1Y |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Novosphingobium sp. PP1Y |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.9 | 8.8 | the enzyme retains 47% and 36% of activity at pH 7.9 and 8.8 | Novosphingobium sp. PP1Y |