Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris AorhaA | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
rutin | pH 5.0, 50°C | Aspergillus oryzae | |
0.53 | - |
naringin | pH 5.0, 50°C | Aspergillus oryzae | |
1.4 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 5.0, 50°C | Aspergillus oryzae | |
1.71 | - |
hesperidin | pH 5.0, 50°C | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-rhamnopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
hesperidin + H2O | 134% of the activity compared to 4-nitrophenyl-alpha-L-rhamnopyranoside | Aspergillus oryzae | hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose | - |
? | |
naringin + H2O | 26% of the activity compared to 4-nitrophenyl-alpha-L-rhamnopyranoside | Aspergillus oryzae | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
rutin + H2O | 68% of the activity compared to 4-nitrophenyl-alpha-L-rhamnopyranoside | Aspergillus oryzae | isoquercitrin + alpha-L-rhamnose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AorhaA | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 75 | 45°C: about 60% of maximal activity, 75°C: about 45% of maximal activity | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, enzyme retained more than 90% of its initial activity. It retains more than 80% of its initial activity after incubation for 2 h and does not further lose activity after treatment for 24 h | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
210 | - |
naringin | pH 5.0, 50°C | Aspergillus oryzae | |
550 | - |
rutin | pH 5.0, 50°C | Aspergillus oryzae | |
1080 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 5.0, 50°C | Aspergillus oryzae | |
2810 | - |
hesperidin | pH 5.0, 50°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | pH 3.0: about 60% of maximal activity, pH 8.0: about 50% of maximal activity | Aspergillus oryzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
400 | - |
naringin | pH 5.0, 50°C | Aspergillus oryzae | |
780 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 5.0, 50°C | Aspergillus oryzae | |
1410 | - |
rutin | pH 5.0, 50°C | Aspergillus oryzae | |
1640 | - |
hesperidin | pH 5.0, 50°C | Aspergillus oryzae |