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Literature summary for 3.2.1.40 extracted from
- Characterization of a alpha-L-rhamnosidase from Bacteroides thetaiotaomicron with high catalytic efficiency of epimedin C (2018), Bioorg. Chem., 81, 461-467 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacteroides thetaiotaomicron |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 5 mM, 92% loss of activity. 1 mM, 83% loss of activity | Bacteroides thetaiotaomicron |  |
| Fe2+ | 5 mM, 12% loss of activity | Bacteroides thetaiotaomicron | |
| Fe3+ | 5 mM, 12% loss of activity | Bacteroides thetaiotaomicron | |
| Hg2+ | 1 mM, 99% loss of activity | Bacteroides thetaiotaomicron | |
| Ni2+ | 5 mM, 28% loss of activity | Bacteroides thetaiotaomicron | |
| Zn2+ | 5 mM, 32% loss of activity. 1 mM, 55% loss of activity | Bacteroides thetaiotaomicron | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.87 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 55°C | Bacteroides thetaiotaomicron | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 1.3fold activation | Bacteroides thetaiotaomicron | |
| Mn2+ | 5 mM, 1.26fold activation | Bacteroides thetaiotaomicron | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83400 | - |
SDS-PAGE | Bacteroides thetaiotaomicron |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides thetaiotaomicron | Q8A904 | - |
- |
| Bacteroides thetaiotaomicron VPI-5482 | Q8A904 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacteroides thetaiotaomicron |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-rhamnopyranoside + H2O | no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl beta-D-galactopyranoside | Bacteroides thetaiotaomicron | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
| 4-nitrophenyl alpha-L-rhamnopyranoside + H2O | no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl beta-D-galactopyranoside | Bacteroides thetaiotaomicron VPI-5482 | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
| epimedin C + H2O | - |
Bacteroides thetaiotaomicron | icariin + alpha-L-rhamnopyranose | - |
? | |
| epimedin C + H2O | - |
Bacteroides thetaiotaomicron VPI-5482 | icariin + alpha-L-rhamnopyranose | - |
? | |
| hesperidin + H2O | - |
Bacteroides thetaiotaomicron | hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose | - |
? | |
| hesperidin + H2O | - |
Bacteroides thetaiotaomicron VPI-5482 | hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose | - |
? | |
| additional information | no the enzyme exhibits high selectivity to cleave the alpha-1,2 and alpha-1,6 glycosidic bond between rhamnoside and rhamnoside, rhamnoside and glycoside | Bacteroides thetaiotaomicron | ? | - |
? | |
| additional information | no the enzyme exhibits high selectivity to cleave the alpha-1,2 and alpha-1,6 glycosidic bond between rhamnoside and rhamnoside, rhamnoside and glycoside | Bacteroides thetaiotaomicron VPI-5482 | ? | - |
? | |
| rutin + H2O | - |
Bacteroides thetaiotaomicron | isoquercitrin + alpha-L-rhamnose | - |
? | |
| rutin + H2O | - |
Bacteroides thetaiotaomicron VPI-5482 | isoquercitrin + alpha-L-rhamnose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 83400, SDS-PAGE | Bacteroides thetaiotaomicron |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BtRha | - |
Bacteroides thetaiotaomicron |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
hydrolysis of epimedin C, hydrolysis of 4-nitrophenyl alpha-L-rhamnopyranoside | Bacteroides thetaiotaomicron |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | 40°C: about 50% of maximal activity, 60°C: about 80% of maximal activity, hydrolysis of 4-nitrophenyl alpha-L-rhamnopyranoside | Bacteroides thetaiotaomicron |
| 50 | 60 | 50°C: about 65% of maximal activity, 60°C: about 75% of maximal activity, hydrolysis of epimedin C | Bacteroides thetaiotaomicron |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
4 h, residual activity is more than 50% | Bacteroides thetaiotaomicron |
| 50 | - |
half-life: 1 h | Bacteroides thetaiotaomicron |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1743.29 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 55°C | Bacteroides thetaiotaomicron | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bacteroides thetaiotaomicron |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | stable in the pH range 5.0-8.0 at 45°C for 4 h over 60% | Bacteroides thetaiotaomicron |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bacteroides thetaiotaomicron | the specific activity of rhamnosidase was 0.57 U/mg in LB medium with 0.1 mM isopropyl beta-D-thiogalactoside induction at 28 °C for 8 h | up |
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