Application | Comment | Organism |
---|---|---|
synthesis | immobilization of recombinant enzyme on Ca2+ alginate beads. Immobilization enables its reutilization up to 9 hydrolysis batches without an appreciable loss in activity | Thermoclostridium stercorarium |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermoclostridium stercorarium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoclostridium stercorarium | Q9S3L0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
naringin + H2O | the free and immobilized enzymes achieve 76% and 67% hydrolysis of the naringin in Kinnow juice, respectively | Thermoclostridium stercorarium | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
free enzyme | Thermoclostridium stercorarium |
60 | - |
enzyme immobilized in Ca2+ alginate beads | Thermoclostridium stercorarium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
free enzyme, less than 50% loss of activityafter 3 h | Thermoclostridium stercorarium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
free enzyme | Thermoclostridium stercorarium |
7.5 | - |
enzyme immobilized in Ca2+ alginate beads | Thermoclostridium stercorarium |