Application | Comment | Organism |
---|---|---|
nutrition | alpha-L-rhamnosidases are used in wine production | Thermomicrobia bacterium |
Cloned (Comment) | Organism |
---|---|
gene rhmA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Thermomicrobia bacterium |
gene rhmB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Thermomicrobia bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | about 500 mM D-glucose concentration in grape juice inhibits RhmA by 39%; about 500 mM D-glucose concentration in grape juice inhibits RhmB by 38% | Thermomicrobia bacterium | |
ethanol | at 12% v/v, 51% inhibition of RhmB; at 12% v/v, 60% inhibition of RhmA | Thermomicrobia bacterium | |
L-rhamnose | complete inhibition, competitive product inhibition; complete inhibition, competitive product inhibition | Thermomicrobia bacterium | |
Zn2+ | 30-35% inhibition at 2 mM, reversible by 10 mM EDTA | Thermomicrobia bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Thermomicrobia bacterium | |
0.46 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 7.5, 50°C, recombinant RhmA | Thermomicrobia bacterium | |
0.66 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 7.0, 50°C, recombinant RhmB | Thermomicrobia bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by EDTA, Mg2+, Ca2+, and Mn2+ on RhmB | Thermomicrobia bacterium | |
additional information | no effect by EDTA, Zn2+, Mg2+, Ca2+, and Mn2+ on RhmA | Thermomicrobia bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
104000 | - |
2 * 104000, recombinant enzyme, SDS-PAGE | Thermomicrobia bacterium |
107000 | - |
2 * 107000, recombinant enzyme, SDS-PAGE | Thermomicrobia bacterium |
210000 | - |
recombinant enzyme, gel filtration | Thermomicrobia bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomicrobia bacterium | Q6RCI8 | gene rhmB, isozyme RhmB | - |
Thermomicrobia bacterium | Q6RCI9 | gene rhmA, isozyme RhmA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 63fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography | Thermomicrobia bacterium |
recombinant enzyme 85fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography | Thermomicrobia bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
108.9 | - |
purified recombinant enzyme | Thermomicrobia bacterium |
263.9 | - |
purified recombinant enzyme | Thermomicrobia bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-rhamnopyranoside + H2O | - |
Thermomicrobia bacterium | 4-nitrophenol + alpha-L-rhamnose | - |
? | |
hesperidin + H2O | cleavage of the alpha-1,6-linkage | Thermomicrobia bacterium | ? + alpha-L-rhamnose | - |
? | |
additional information | no activity with quercitrin | Thermomicrobia bacterium | ? | - |
? | |
additional information | no activity with rutin and quercitrin | Thermomicrobia bacterium | ? | - |
? | |
naringin + H2O | preferred substrate, cleavage of the alpha-1,2-linkage | Thermomicrobia bacterium | ? + alpha-L-rhamnose | - |
? | |
rhamnogalacturonan tetramer + H2O | - |
Thermomicrobia bacterium | ? | - |
? | |
rutin + H2O | low activity, cleavage of the alpha-1,6-linkage | Thermomicrobia bacterium | ? + alpha-L-rhamnose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 104000, recombinant enzyme, SDS-PAGE | Thermomicrobia bacterium |
dimer | 2 * 107000, recombinant enzyme, SDS-PAGE | Thermomicrobia bacterium |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-rhamnosidase A | - |
Thermomicrobia bacterium |
alpha-L-rhamnosidase B | - |
Thermomicrobia bacterium |
RhmA | - |
Thermomicrobia bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermomicrobia bacterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | low activity above | Thermomicrobia bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
completely stable for 24 h at pH 5.0-6.9 | Thermomicrobia bacterium |
40 | - |
loss of 22% activity within 24 h at pH 7.9 | Thermomicrobia bacterium |
60 | - |
20-30% remaining activity after 24 h at pH 5.0-6.9 | Thermomicrobia bacterium |
60 | - |
20-30% remaining activity after 24 h at pH 7.9 | Thermomicrobia bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6.9 | - |
Thermomicrobia bacterium |
7.9 | - |
- |
Thermomicrobia bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7.9 | 50% of maximal activity at pH 4.0 and pH 7.9 | Thermomicrobia bacterium |
5 | 8.7 | 50% of maximal activity at pH 5.0 and pH 8.7 | Thermomicrobia bacterium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.28 | - |
L-rhamnose | pH 7.5, 50°C, recombinant RhmA, versus 4-nitrophenyl alpha-L-rhamnopyranoside | Thermomicrobia bacterium | |
4.65 | - |
L-rhamnose | pH 7.0, 50°C, recombinant RhmB, versus 4-nitrophenyl alpha-L-rhamnopyranoside | Thermomicrobia bacterium |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomicrobia bacterium | sequence calculation | - |
4.5 |
Thermomicrobia bacterium | sequence calculation | - |
4.6 |