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Literature summary for 3.2.1.40 extracted from

  • Manzanares, P.; van den Broeck, H.C.; de Graaff, L.H.; Visser, J.
    Purification and characterization of two different alpha-L-rhamnosidases, RhaA and RhaB, from Aspergillus aculeatus (2001), Appl. Environ. Microbiol., 67, 2230-2234.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.40

Inhibitors

Inhibitors Comment Organism Structure
L-rhamnose competitive Aspergillus aculeatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 p-nitrophenyl-alpha-L-rhamnopyranoside pH 4.5, 30°C Aspergillus aculeatus
2.8
-
 p-nitrophenyl-alpha-L-rhamnopyranoside pH 4.5, 30°C Aspergillus aculeatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular growth on hesperidin Aspergillus aculeatus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 x * 85000, SDS-PAGE Aspergillus aculeatus
92000
-
 x * 92000, SDS-PAGE Aspergillus aculeatus

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus Q9C1M9 isozyme RhaA, groth on hesperidin
-
Aspergillus aculeatus Q9HFW5 isozyme RhaB, growth on hesperidin
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein only N-glycosylation, about 15% carbohydrate Aspergillus aculeatus
glycoprotein only N-glycosylation, about 24% carbohydrate Aspergillus aculeatus

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus aculeatus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
 pH 4.5, 30°C Aspergillus aculeatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hesperidin + H2O
-
 Aspergillus aculeatus L-rhamnose + ?
-
 ?
additional information no substrate: quercitrin Aspergillus aculeatus ?
-
 ?
naringin + H2O
-
 Aspergillus aculeatus L-rhamnose + ?
-
 ?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Aspergillus aculeatus p-nitrophenol + alpha-L-rhamnopyranose
-
 ?
rutin + H2O
-
 Aspergillus aculeatus L-rhamnose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 85000, SDS-PAGE Aspergillus aculeatus
? x * 92000, SDS-PAGE Aspergillus aculeatus
More N-terminal amino acid sequence Aspergillus aculeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 20 h, 80% residual stability Aspergillus aculeatus
30
-
 20 h, 90% residual stability Aspergillus aculeatus
40
-
 4 h, stable Aspergillus aculeatus
55
-
 4 h, 75% residual stability Aspergillus aculeatus
55
-
 4 h, 87% residual stability Aspergillus aculeatus
65
-
 4 h, 55% residual stability Aspergillus aculeatus
65
-
 4 h, 60% residual stability Aspergillus aculeatus
75
-
 4 h, 50% residual stability Aspergillus aculeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Aspergillus aculeatus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 5
-
 Aspergillus aculeatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5
-
 Aspergillus aculeatus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5
-
 L-rhamnose pH 4.5, 30°C Aspergillus aculeatus
4.2
-
 L-rhamnose pH 4.5, 30°C Aspergillus aculeatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus aculeatus shows microheterogenity upon isoelectric focusing
-
 additional information
Aspergillus aculeatus calculated from amino acid sequence
-
 6
Aspergillus aculeatus isoelectric focusing
-
 6