Any feedback?
Literature summary for 3.2.1.40 extracted from
- Purification and characterization of intracellular alpha-L-rhamnosidase from Pseudomonas paucimobilis FP2001 (2000), Arch. Microbiol., 173, 65-70.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Sphingomonas paucimobilis |  |
| Cd2+ | - |
Sphingomonas paucimobilis | |
| Cu2+ | - |
Sphingomonas paucimobilis | |
| EDTA | - |
Sphingomonas paucimobilis | |
| EGTA | - |
Sphingomonas paucimobilis | |
| Pb2+ | - |
Sphingomonas paucimobilis | |
| Zn2+ | - |
Sphingomonas paucimobilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
hesperidin | - |
Sphingomonas paucimobilis | |
| 0.07 | - |
proscillaridin A | - |
Sphingomonas paucimobilis | |
| 0.13 | - |
rutin | - |
Sphingomonas paucimobilis | |
| 0.17 | - |
naringin | - |
Sphingomonas paucimobilis | |
| 0.88 | - |
saikosaponin C | - |
Sphingomonas paucimobilis | |
| 0.89 | - |
quercitrin | - |
Sphingomonas paucimobilis | |
| 1.18 | - |
p-nitrophenyl-alpha-L-rhamnopyranoside | - |
Sphingomonas paucimobilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at 1 mM, serves as potential cofactor | Sphingomonas paucimobilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 112000 | - |
gel filtration, SDS-PAGE | Sphingomonas paucimobilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-rhamnoside + H2O | Sphingomonas paucimobilis | alpha-L-rhamnose in glycolipids or glycosides | alpha-L-rhamnose + ? | - |
? | |
| alpha-L-rhamnoside + H2O | Sphingomonas paucimobilis FP2001 | alpha-L-rhamnose in glycolipids or glycosides | alpha-L-rhamnose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas paucimobilis | - |
FP2001 | - |
| Sphingomonas paucimobilis FP2001 | - |
FP2001 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sphingomonas paucimobilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months, no loss of activity | Sphingomonas paucimobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O | - |
Sphingomonas paucimobilis | 4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose | - |
? | |
| 4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O | - |
Sphingomonas paucimobilis FP2001 | 4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose | - |
? | |
| alpha-L-rhamnoside + H2O | alpha-L-rhamnose in glycolipids or glycosides | Sphingomonas paucimobilis | alpha-L-rhamnose + ? | - |
? | |
| alpha-L-rhamnoside + H2O | alpha-L-rhamnose in glycolipids or glycosides | Sphingomonas paucimobilis FP2001 | alpha-L-rhamnose + ? | - |
? | |
| hesperidin + H2O | - |
Sphingomonas paucimobilis | hesperetin 7-glucoside + alpha-L-rhamnose | - |
? | |
| hesperidin + H2O | - |
Sphingomonas paucimobilis FP2001 | hesperetin 7-glucoside + alpha-L-rhamnose | - |
? | |
| naringin + H2O | - |
Sphingomonas paucimobilis | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
| naringin + H2O | - |
Sphingomonas paucimobilis FP2001 | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
| p-nitrophenyl alpha-L-rhamnoside + H2O | - |
Sphingomonas paucimobilis | p-nitrophenol + alpha-L-rhamnose | - |
? | |
| p-nitrophenyl alpha-L-rhamnoside + H2O | - |
Sphingomonas paucimobilis FP2001 | p-nitrophenol + alpha-L-rhamnose | - |
? | |
| p-nitrophenyl-alpha-L-rhamnopyranoside + H2O | - |
Sphingomonas paucimobilis | p-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
| proscillaridin A + H2O | - |
Sphingomonas paucimobilis | pyranosyl 3,14-dihydroxybufa-4,20,22-trienolid + alpha-L-rhamnose | - |
? | |
| quercitrin + H2O | - |
Sphingomonas paucimobilis | quercetin + L-rhamnose | - |
? | |
| rutin + H2O | - |
Sphingomonas paucimobilis | 3-glycosylquercetin + alpha-L-rhamnose | - |
? | |
| saikosaponin C + H2O | - |
Sphingomonas paucimobilis | ? + alpha-L-rhamnose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 112000, SDS-PAGE | Sphingomonas paucimobilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Sphingomonas paucimobilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | - |
Sphingomonas paucimobilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 8 | 40 | stable below 40°C for 1 h | Sphingomonas paucimobilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Sphingomonas paucimobilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Sphingomonas paucimobilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Sphingomonas paucimobilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
