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Literature summary for 3.2.1.40 extracted from
- Rhamnogalacturonan alpha-L-rhamnopyranohydrolase. A novel enzyme specific for the terminal nonreducing rhamnosyl unit in rhamnogalacturonan regions of pectin (1994), Plant Physiol., 106, 241-250.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Sodium acetate | above 0.3 M , at pH 5 | Aspergillus aculeatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
gel filtration, SDS-PAGE | Aspergillus aculeatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-rhamnoside + H2O | Aspergillus aculeatus | alpha-L-rhamnose in glycolipids or glycosides | alpha-L-rhamnose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus aculeatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus aculeatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Aspergillus aculeatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O | - |
Aspergillus aculeatus | 4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose | - |
? | |
| alpha-L-rhamnoside + H2O | alpha-L-rhamnose in glycolipids or glycosides | Aspergillus aculeatus | alpha-L-rhamnose + ? | - |
? | |
| hesperidin + H2O | - |
Aspergillus aculeatus | hesperetin 7-glucoside + alpha-L-rhamnose | - |
? | |
| high-methoxyl pectin + H2O | - |
Aspergillus aculeatus | pyranoside + alpha-L-rhamnose | - |
? | |
| naringin + H2O | - |
Aspergillus aculeatus | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
| p-nitrophenyl alpha-L-rhamnoside + H2O | - |
Aspergillus aculeatus | p-nitrophenol + alpha-L-rhamnose | - |
? | |
| polygalacturonic acid + H2O | - |
Aspergillus aculeatus | D-galacturonic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | similar enzyme: RG-rhamnohydrolase | Aspergillus aculeatus |
| pnp-rhamnohydrolase | - |
Aspergillus aculeatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus aculeatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
in McIlvaine buffer, pH 5, after 30 min, 50% loss of activity | Aspergillus aculeatus |
| 50 | - |
in sodium acetate buffer, pH 5, after 1 h no loss of activity at | Aspergillus aculeatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Aspergillus aculeatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Aspergillus aculeatus |
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Release 2023.1 (January 2023)
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