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Literature summary for 3.2.1.40 extracted from

  • Bourbouze, R.; Percheron, F.; Courtois, J.E.
    alpha-L-Rhamnosidase from Fagopyrum esculentum: purification and some properties (1976), Eur. J. Biochem., 63, 331-337.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
6-deoxy-D-glucose competitive Fagopyrum esculentum
alpha-L-rhamnose competitive Fagopyrum esculentum
L-Lyxose competitive Fagopyrum esculentum
methyl-alpha-D-mannoside competitive Fagopyrum esculentum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
p-nitrophenyl-alpha-L-rhamnoside
-
Fagopyrum esculentum
2.2
-
6-O-alpha-L-rhamnosyl-D-glucopyranose
-
Fagopyrum esculentum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
disc gel electrophoresis, gel filtration Fagopyrum esculentum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-L-rhamnoside + H2O Fagopyrum esculentum alpha-L-rhamnose in glycolipids or glycosides alpha-L-rhamnose + ?
-
?

Organism

Organism UniProt Comment Textmining
Fagopyrum esculentum
-
buckwheat
-

Purification (Commentary)

Purification (Comment) Organism
saracen corn Fagopyrum esculentum

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Fagopyrum esculentum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-O-alpha-L-rhamnosyl-D-glucopyranose + H2O rutinose Fagopyrum esculentum alpha-L-rhamnose + D-glucose
-
?
alpha-L-rhamnoside + H2O alpha-L-rhamnose in glycolipids or glycosides Fagopyrum esculentum alpha-L-rhamnose + ?
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
-
Fagopyrum esculentum p-nitrophenol + alpha-L-rhamnose
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
Fagopyrum esculentum p-nitrophenol + alpha-L-rhamnopyranose
-
?