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Literature summary for 3.2.1.4 extracted from

  • Mohammad, S.F.; Feng, Y.; Yang, G.
    Optimization of cell culture and cell disruption processes to enhance the production of thermophilic cellulase FnCel5A in E.coli using response surface methodology (2019), PLoS ONE, 14, e0210595 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Application

Application Comment Organism
biofuel production the enzyme can be useful for production of bioethanol and biofuel Fervidobacterium nodosum

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of FnCel5A in Escherichia coli strain BL21, method evaluation and optimization for overproduction and up-scaling to be used in production of bioethanol and biofuel Fervidobacterium nodosum

Organism

Organism UniProt Comment Textmining
Fervidobacterium nodosum D4PEB3
-
-
Fervidobacterium nodosum ATCC 35602 D4PEB3
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Fervidobacterium nodosum DSM 5306 D4PEB3
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-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 recombinant crude enzyme, 5.772 U/ml Fervidobacterium nodosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O high activity Fervidobacterium nodosum D-glucose + ?
-
 ?
barley beta-glucan + H2O high activity Fervidobacterium nodosum ATCC 35602 D-glucose + ?
-
 ?
barley beta-glucan + H2O high activity Fervidobacterium nodosum DSM 5306 D-glucose + ?
-
 ?
carboxymethyl cellulose + H2O high activity Fervidobacterium nodosum D-glucose + ?
-
 ?
carboxymethyl cellulose + H2O high activity Fervidobacterium nodosum ATCC 35602 D-glucose + ?
-
 ?
carboxymethyl cellulose + H2O high activity Fervidobacterium nodosum DSM 5306 D-glucose + ?
-
 ?
cellulose + H2O enzyme FnCel5A catalyzes the hydrolysis of cellulose to glucose Fervidobacterium nodosum D-glucose + ?
-
 ?
cellulose + H2O enzyme FnCel5A catalyzes the hydrolysis of cellulose to glucose Fervidobacterium nodosum ATCC 35602 D-glucose + ?
-
 ?
cellulose + H2O enzyme FnCel5A catalyzes the hydrolysis of cellulose to glucose Fervidobacterium nodosum DSM 5306 D-glucose + ?
-
 ?
additional information the enzyme shows high affinity for carboxymethyl cellulose, but is also active with other substrates like beta-1,4-linked polysaccharides, including xyloglucan, glucomannan, beta-glucan, lichenin, and galactomannan Fervidobacterium nodosum ?
-
 ?
additional information the enzyme shows high affinity for carboxymethyl cellulose, but is also active with other substrates like beta-1,4-linked polysaccharides, including xyloglucan, glucomannan, beta-glucan, lichenin, and galactomannan Fervidobacterium nodosum ATCC 35602 ?
-
 ?
additional information the enzyme shows high affinity for carboxymethyl cellulose, but is also active with other substrates like beta-1,4-linked polysaccharides, including xyloglucan, glucomannan, beta-glucan, lichenin, and galactomannan Fervidobacterium nodosum DSM 5306 ?
-
 ?

Synonyms

Synonyms Comment Organism
endoglucanase
-
 Fervidobacterium nodosum
FnCel5A
-
 Fervidobacterium nodosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Fervidobacterium nodosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified recombinant enzyme, half-life is 48 h Fervidobacterium nodosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Fervidobacterium nodosum

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 5, GH5 Fervidobacterium nodosum