Cloned (Comment) | Organism |
---|---|
gene 168cel5 or eglS, recombinant expression of the enzyme, in an expression construct comprising DNA sequences encoding P43 promoter, signal peptide of Bacillus subtilis nprB, and mature Bacillus subtilis strain 168 Cel5 residues 30-499, in Paenibacillus sp. strain CAA11 | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | cellulolytic activity of Paenibacillus sp. strain CAA11 is significantly enhanced by expressing a heterologous endoglucanase 168Cel5 from Bacillus subtilis (DNA sequences encoding P43 promoter, signal peptide of Bacillus subtilis nprB, and mature Bacillus subtilis strain 168 Cel5 residues 30-499) under both aerobic and anaerobic conditions. The strain harboring the 168cel5 gene reveals 2fold bigger halo zone on Congo-red plate and 1.75fold more aerobic cellulose utilization in liquid medium compared with the negative control. Under anaerobic conditions, the recombinant strain expressing 168Cel5 consumes 1.83fold more cellulose (5.10 g/l) and produces 5fold more ethanol (0.65 g/l) along with 5fold more total acids (1.6 g/l) compared with the control, resulting in 2.73fold higher yields. Optimal growth of the engineered strain at 37°C, evaluation of optimal growth conditions, overview | Paenibacillus sp. CAA11 |
additional information | cellulolytic activity of Paenibacillus sp. strain CAA11 is significantly enhanced by expressing a heterologous endoglucanase 168Cel5 from Bacillus subtilis under both aerobic and anaerobic conditions. The strain harboring the 168cel5 gene reveals 2fold bigger halo zone on Congo-red plate and 1.75fold more aerobic cellulose utilization in liquid medium compared with the negative control. Under anaerobic conditions, the recombinant strain expressing 168Cel5 consumes 1.83fold more cellulose (5.10 g/l) and produces 5fold more ethanol (0.65 g/l) along with 5fold more total acids (1.6 g/l) compared with the control, resulting in 2.73fold higher yields | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Bacillus subtilis | - |
- |
extracellular | the enzyme is secreted | Paenibacillus sp. CAA11 | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P10475 | - |
- |
Bacillus subtilis 168 | P10475 | - |
- |
Paenibacillus sp. CAA11 | A0A2S0UGE4 | isolated from sediment | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Paenibacillus sp. strain CAA11 is able to utilize cellulose as a sole carbon source | Paenibacillus sp. CAA11 | - |
Synonyms | Comment | Organism |
---|---|---|
168cel5 | - |
Bacillus subtilis |
DCC85_10145 | locus name | Paenibacillus sp. CAA11 |
EglS | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | Paenibacillus sp. strain CAA11 secretes cellulolytic enzymes by its own secretion system and produces ethanol as well as short-chain organic acids (formic acid, acetic acid, lactic acid) from cellulose with rel. low activity | Paenibacillus sp. CAA11 |