Cloned (Comment) | Organism |
---|---|
sequence comparisons, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115 | Aspergillus glaucus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Aspergillus glaucus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates about 20% at 1 mM | Aspergillus glaucus | |
Ca2+ | activates about 20% at 1 mM | Aspergillus glaucus | |
Co2+ | activates 2fold at 1 mM | Aspergillus glaucus | |
Cu2+ | activates 1.5fold at 1 mM | Aspergillus glaucus | |
Mg2+ | activates about 30% at 1 mM | Aspergillus glaucus | |
Mn2+ | activates about 20% at 1 mM | Aspergillus glaucus | |
NaCl | the enzyme shows higher activity at NaCl concentrations from 1.0 to 4.0 M | Aspergillus glaucus | |
Ni2+ | activates about 20% at 1 mM | Aspergillus glaucus | |
Zn2+ | activates about 25% at 1 mM | Aspergillus glaucus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus glaucus | A0A2P1G1K5 | i.e. Eurotium herbariorum | - |
Aspergillus glaucus CCHA | A0A2P1G1K5 | i.e. Eurotium herbariorum | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has two potential N-glycosylation sites at the N-terminus (positions 37 and 60) and 60 potential O-glycosylation sites at the C-terminus | Aspergillus glaucus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography to homogeneity | Aspergillus glaucus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
297.15 | - |
purified recombinant His-tagged enzyme, pH 5.0, 55°C, substrate carboxymethyl cellulose | Aspergillus glaucus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Aspergillus glaucus | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Aspergillus glaucus CCHA | ? | - |
? | |
additional information | hydrolysis of rice and corn straws | Aspergillus glaucus | ? | - |
? | |
additional information | hydrolysis of rice and corn straws | Aspergillus glaucus CCHA | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, about, recombinant enzyme, SDS-PAGE, x * 52800, about, sequence calculation | Aspergillus glaucus |
Synonyms | Comment | Organism |
---|---|---|
AgCMCase | - |
Aspergillus glaucus |
endoglucanase | - |
Aspergillus glaucus |
GH5 cellulase | - |
Aspergillus glaucus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme | Aspergillus glaucus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | over 60% of maximal activity within this range, profile overview | Aspergillus glaucus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recombinant enzyme | Aspergillus glaucus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | over 30% of maximal activity within this range, profile overview | Aspergillus glaucus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | purified recombinant His-tagged enzyme, over 50% activity remaining at pH 4.0-8.5, 40% at pH 9.0-10.0 | Aspergillus glaucus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus glaucus | sequence calculation | - |
5.2 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 5, GH5 | Aspergillus glaucus |
additional information | the unique GH5 cellulase member in the extreme halotolerant fungus Aspergillus glaucus CCHA is an endoglucanase with multiple tolerance to salt, alkali and heat. Homology modeling of AgCMCase | Aspergillus glaucus |