Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Khalili Ghadikolaei, K.; Gharechahi, J.; Haghbeen, K.; Akbari Noghabi, K.; Hosseini Salekdeh, G.; Shahbani Zahiri, H.
    A cold-adapted endoglucanase from camel rumen with high catalytic activity at moderate and low temperatures an anomaly of truly cold-adapted evolution in a mesophilic environment (2018), Extremophiles, 22, 315-326 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
cloned from camel rumen metagenom, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
acetone
-
 uncultured bacterium
Benzene
-
 uncultured bacterium
chloroform
-
 uncultured bacterium
Cyclohexane
-
 uncultured bacterium
ethanol
-
 uncultured bacterium
guanidinium hydrochloride 72.2% at 3% uncultured bacterium
isobutanol
-
 uncultured bacterium
Isopropanol
-
 uncultured bacterium
methanol
-
 uncultured bacterium
additional information organic solvents at 10-20% uncultured bacterium
n-hexane
-
 uncultured bacterium
PMSF 46.7% at 5 mM uncultured bacterium
SDS complete inhibition at 0.1% uncultured bacterium
Toluene
-
 uncultured bacterium
Tween 20 31% inhibition at 20% uncultured bacterium
Urea 82.3% inhibition at 10%, 42.3% at 7%, and 24.3 at 5% uncultured bacterium
Zn2+ about 60-80% inhibition at 5-10 mM uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Vmax and Km are 339 U/mg and 2.57 mg/ml, respectively, Michaelis-Menten kinetics and thermodynamic analysis, overview uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates slightly at 10 mM uncultured bacterium
Co2+ activates at 5-10 mM uncultured bacterium
Mn2+ activates at 5-10 mM uncultured bacterium
Na+ activates at 5 mM, not at 10 mM uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium A0A1W5YNV1 isolated from camel rumen
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 uncultured bacterium ?
-
 ?
additional information no activity with starch uncultured bacterium ?
-
 ?
oat spelt xylan + H2O
-
 uncultured bacterium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46500, about, sequence calculation uncultured bacterium

Synonyms

Synonyms Comment Organism
CelCM3
-
 uncultured bacterium
cellulase CM3
-
 uncultured bacterium
endoglucanase
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 optimum temperature is 30°C, over 60% of maximal activity in the range of 10-50°C, 13% at 60°C, no activity at 70-80°C, profile overview uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 45 the enzyme has an estimated low melting temperature of 45°C, about 50% activity remaining at 4°C, it is identified to be cold-adapted uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
3 9 activity range, over 50% of maximal activity at pH 4.0-7.5, profile overview uncultured bacterium