Cloned (Comment) | Organism |
---|---|
gene ge40, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic structure of the cloning construct, cloning with a signal peptide sequence derived from the endoxylanse (EC 3.2.1.8) encoding gene GtxynA1 from Geobacillus thermodenitrificans strain T12, overview. Recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) (under control of the lac operator) and in Geobacillus thermodenitrificans strain T12 | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A0A291I5R3 | - |
- |
Geobacillus thermodenitrificans T12 | A0A291I5R3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyethyl cellulose + H2O | - |
Geobacillus thermodenitrificans | ? | - |
? | |
2-hydroxyethyl cellulose + H2O | - |
Geobacillus thermodenitrificans T12 | ? | - |
? | |
barley beta-glucan + H2O | - |
Geobacillus thermodenitrificans | ? | - |
? | |
barley beta-glucan + H2O | - |
Geobacillus thermodenitrificans T12 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Geobacillus thermodenitrificans | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Geobacillus thermodenitrificans T12 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endoglucanase | - |
Geobacillus thermodenitrificans |
GE40 | - |
Geobacillus thermodenitrificans |
GE40 endoglucanase | - |
Geobacillus thermodenitrificans |
T12-GE40 | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Geobacillus thermodenitrificans |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 5, GH5 | Geobacillus thermodenitrificans |