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Literature summary for 3.2.1.4 extracted from

  • Prajapati, B.P.; Kumar Suryawanshi, R.; Agrawal, S.; Ghosh, M.; Kango, N.
    Characterization of cellulase from Aspergillus tubingensis NKBP-55 for generation of fermentable sugars from agricultural residues (2018), Biores. Technol., 250, 733-740 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 40% activation at 5 mM Aspergillus tubingensis

Inhibitors

Inhibitors Comment Organism Structure
acetonitril 11.4% inhibition at 5% Aspergillus tubingensis
benzol 96.2% inhibition at 5% Aspergillus tubingensis
butanol 10.9% inhibition at 5% Aspergillus tubingensis
Fe2+ 10% inhibition at 5 mM Aspergillus tubingensis
Hg2+ 73.5% inhibition at 5 mM Aspergillus tubingensis
additional information below 10% inhibition by propan-2-ol, ethanol, methanol, tert-amyl alcohol, acetone, chloroform, hexane, toluene, ethyl acetate, glycerol, Tween 80, and Tween 20 at 5%, and by Ca2+, Mg2+, Zn2+, (NH4)2SO4, urea, NaN2, and EDTA at 5 mM Aspergillus tubingensis
Na+ 23% inhibition at 5 mM Aspergillus tubingensis
SDS 42% inhibition at 5% Aspergillus tubingensis
Triton X-100 19.7% inhibition at 5% Aspergillus tubingensis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 15% activation at 5 mM Aspergillus tubingensis
Mn2+ 30% activation at 5 mM Aspergillus tubingensis

Organism

Organism UniProt Comment Textmining
Aspergillus tubingensis
-
 isolated from cow dung compost
-
Aspergillus tubingensis NKBP-55
-
 isolated from cow dung compost
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 3.7fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration, to homogeneity Aspergillus tubingensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
97.95
-
 purified native enzyme, pH 5.0, 70°C Aspergillus tubingensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Aspergillus tubingensis ?
-
 ?
carboxymethyl cellulose + H2O
-
 Aspergillus tubingensis NKBP-55 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Aspergillus tubingensis

Synonyms

Synonyms Comment Organism
Cat 1
-
 Aspergillus tubingensis
CMCase
-
 Aspergillus tubingensis
endo-glucanase
-
 Aspergillus tubingensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Aspergillus tubingensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 purified native enzyme, pH 5.0, the enzyme retains more than 50% activity after incubation at 65°C and 70°C for 180 min, no major loss in activity at 50°C Aspergillus tubingensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus tubingensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5 purified native enzyme, 60% activity remaining at pH 3.0 after 24 h, completely stable at pH 5.0 Aspergillus tubingensis