Activating Compound | Comment | Organism | Structure |
---|---|---|---|
butanol | butanol-tolerant endoglucanase, enzyme activity is 1.18fold increased in 5% butanol solution compared to butanol-free solution. Melting point temperature is 2.5°C higher in 5% butanol solution (50°C) than in butanol-free solution (47.5°C). At 45-60°C, the Gbbs-free energy change, DELTAG, is higher in 5% butanol solution than that in butanol-free solution | Rhodococcus sp. | |
additional information | no effect by DTT and EDTA at 10 mM | Rhodococcus sp. |
Application | Comment | Organism |
---|---|---|
synthesis | the cold active butanol-tolerant endoglucanase is valuable for biobutanol production by a simultaneous saccharification and fermentation process | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 14% inhibition at 10 mM | Rhodococcus sp. | |
Cu2+ | 22% inhibition at 10 mM | Rhodococcus sp. | |
additional information | no effect by DTT and EDTA at 10 mM | Rhodococcus sp. | |
Pb2+ | 65% inhibition at 10 mM | Rhodococcus sp. | |
Zn2+ | 74% inhibition at 10 mM | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamic kinetics of the endoglucanase at different concentrations of butanol | Rhodococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no or poor effect by Ca2+, Na+, K+, Mg2+, and Fe2+ | Rhodococcus sp. |
Organic Solvent | Comment | Organism |
---|---|---|
Butanol | butanol-tolerant endoglucanase, enzyme activity is 1.18fold increased in 5% butanol solution compared to butanol-free solution. Melting point temperature is 2.5°C higher in 5% butanol solution (50°C) than in butanol-free solution (47.5°C). At 45-60°C, the Gibbs-free energy change, DELTAG, is higher in 5% butanol solution than that in butanol-free solution. The content of alpha-helix and beta-sheet increases in 5% butanol solution | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | best substrate | Rhodococcus sp. | ? | - |
? | |
cellulose + H2O | filter paper, 18% activity compared to carboxymethyl cellulose | Rhodococcus sp. | ? | - |
? | |
additional information | no activity with Avicel, cotton, laminarin, and cellobiose | Rhodococcus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the content of alpha-helix and beta-sheet increases in 5% butanol solution. The increasing of alpha-helix and beta-sheet content leads to higher activity and better thermostability in butanol solution | Rhodococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Rhodococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
cold-active endoglucanase | Rhodococcus sp. |
10 | 70 | and above, activity range, profile overview. Over 70% activity at 30-65°C | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Rhodococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | over 80% of maximal activity within this range | Rhodococcus sp. |