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Literature summary for 3.2.1.4 extracted from

  • Chen, X.; Li, W.; Ji, P.; Zhao, Y.; Hua, C.; Han, C.
    Engineering the conserved and noncatalytic residues of a thermostable beta-1,4-endoglucanase to improve specific activity and thermostability (2018), Sci. Rep., 8, 2954 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure Thermochaetoides thermophila

Protein Variants

Protein Variants Comment Organism
Y173F 1.9fold increased the enzyme's specific activity. Mutation significantly improves the enzyme’s heat resistance at 80°C and 90°C Thermochaetoides thermophila
Y30F 1.4fold increased the enzyme's specific activity Thermochaetoides thermophila
Y30F/Y173F mutant shows considerably higher stability at elevated temperatures but does not display the increased catalytic efficiency of its single mutant counterparts Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila V9PD44
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
 substrate beta-D-glucan, mutant Y30F/Y173F, pH 4.0, 60°C Thermochaetoides thermophila
0.68
-
 substrate carboxymethylcellulose, mutant Y30F/Y173F, pH 4.0, 60°C Thermochaetoides thermophila
1
-
 substrate beta-D-glucan, wild-type, pH 4.0, 60°C Thermochaetoides thermophila
1.21
-
 substrate beta-D-glucan, mutant Y30F, pH 4.0, 60°C Thermochaetoides thermophila
1.21
-
 substrate carboxymethylcellulose, wild-type, pH 4.0, 60°C Thermochaetoides thermophila
1.35
-
 substrate carboxymethylcellulose, mutant Y30F, pH 4.0, 60°C Thermochaetoides thermophila
1.67
-
 substrate beta-D-glucan, mutant Y173F, pH 4.0, 60°C Thermochaetoides thermophila
1.87
-
 substrate carboxymethylcellulose, mutant Y173F, pH 4.0, 60°C Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucan + H2O
-
 Thermochaetoides thermophila ?
-
 ?
carboxymethylcellulose + H2O
-
 Thermochaetoides thermophila ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 wild-type and mutants Thermochaetoides thermophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80
-
 mutants Y30F and Y173F maintain more than 70% of maximum activity Thermochaetoides thermophila