Cloned (Comment) | Organism |
---|---|
truncated mutants are constructed by deleting five residues from C-termini of endoglucanase catalytic domain. The truncated gene is inserted into the expression vector pET11a (Novagen, Madison, WI). The constructed plasmids are introduced into Escherichia coli strain BL21(DE3) | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58925 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
PH1171 | - |
Pyrococcus horikoshii |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme involved in the degradation of beta-glucan cellulose biomass acting through hydrolysis of the unbranched beta-1,4-linked glucose homopolymer | Pyrococcus horikoshii |