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Literature summary for 3.2.1.4 extracted from

  • Lee, J.P.; Kim, Y.A.; Kim, S.K.; Kim, H.
    Characterization of a multimodular endo-beta-1,4-glucanase (Cel9K) from Paenibacillus sp. X4 with a potential additive for saccharification (2018), J. Microbiol. Biotechnol., 28, 588-596 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Application

Application Comment Organism
degradation addition of Cel9K to a commercial enzyme set (Celluclast 1.5L + Novozym 188) increases the saccharification of the pretreated reed and rice straw powders by 30.4% and 15.9%, respectively Paenibacillus sp. X4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenibacillus sp. X4

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 2 mM, less than 50% of initial activity Paenibacillus sp. X4
Mn2+ 2 mM, less than 50% of initial activity Paenibacillus sp. X4

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 2 mM, more than 150% of initial activity Paenibacillus sp. X4
K+ 2 mM, more than 150% of initial activity Paenibacillus sp. X4
Na+ 2 mM, more than 150% of initial activity Paenibacillus sp. X4

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. X4 MG570051
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O 311% of the activity with carboxymethylcellulose Paenibacillus sp. X4 ?
-
 ?
carboxymethylcellulose + H2O
-
 Paenibacillus sp. X4 ?
-
 ?
lichenan + H2O 157% of the activity with carboxymethylcellulose Paenibacillus sp. X4 ?
-
 ?
additional information low hydrolytic activities, toward birchwood xylan and laminarin Paenibacillus sp. X4 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 103500, calculated, x * 93000, SDS-PAGE, truncated form produced by the proteolytic cleavage of the C-terminus Paenibacillus sp. X4

Synonyms

Synonyms Comment Organism
Cel9K
-
 Paenibacillus sp. X4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenibacillus sp. X4

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
 56% of maximum activity Paenibacillus sp. X4
60
-
 67% of maximum activity Paenibacillus sp. X4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life 59 min Paenibacillus sp. X4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Paenibacillus sp. X4

pI Value

Organism Comment pI Value Maximum pI Value
Paenibacillus sp. X4 calculated
-
 4.8