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Literature summary for 3.2.1.4 extracted from
- Genetic and structural characterization of a thermo-tolerant, cold-active, and acidic endo-beta-1,4-glucanase from Antarctic springtail, Cryptopygus antarcticus (2017), J. Agric. Food Chem., 65, 1630-1640 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | under acidic conditions at 50°C, the enzyme is effective in digesting the green algae Ulva pertusa | Cryptopygus antarcticus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cryptopygus antarcticus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular modeling of structure | Cryptopygus antarcticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cryptopygus antarcticus | D3GDK4 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.9 | - |
substrate barley beta-glucan, pH 3.5, 40°C | Cryptopygus antarcticus |
| 20.5 | - |
substrate lichenan, pH 3.5, 40°C | Cryptopygus antarcticus |
| 35.8 | - |
substrate carboxymethylcellulose, pH 3.5, 40°C | Cryptopygus antarcticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Cryptopygus antarcticus | ? | - |
? |  |
| carboxymethylcellulose + H2O | - |
Cryptopygus antarcticus | cellobiose + cellotriose + cellotetraose | - |
? | |
| cellohexaose + H2O | - |
Cryptopygus antarcticus | cellotetraose + cellobiose | - |
? | |
| cellopentaose + H2O | - |
Cryptopygus antarcticus | cellotriose + cellobiose | - |
? | |
| cellotetraose + H2O | - |
Cryptopygus antarcticus | 2 cellobiose | - |
? | |
| lichenan + H2O | - |
Cryptopygus antarcticus | ? | - |
? | |
| additional information | no substrates: cellobiose and cellotriose | Cryptopygus antarcticus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Cryptopygus antarcticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
10 | 60% of maximum activity | Cryptopygus antarcticus |
| 80 | - |
40% of maximum activity | Cryptopygus antarcticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
8 h, no loss of activity | Cryptopygus antarcticus |
| 70 | - |
half-life 4 h | Cryptopygus antarcticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Cryptopygus antarcticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 7 | more than 60% of maximum activity | Cryptopygus antarcticus |
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Release 2023.1 (January 2023)
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