Any feedback?
Literature summary for 3.2.1.4 extracted from
- Recombinant thermo-alkali-stable endoglucanase of Myceliopthora thermophila BJA (rMt-egl) Biochemical characteristics and applicability in enzymatic saccharification of agro-residues (2017), Int. J. Biol. Macromol., 104, 107-116 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | enzyme releases high amounts of reducing sugars from wheat bran and corn cobs, being a useful biocatalyst for producing bioethanol and fine chemicals from agroresidues | Thermothelomyces thermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Thermothelomyces thermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure. Residues E234 and E344 display a catalytically important role | Thermothelomyces thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 62% residual activity | Thermothelomyces thermophilus |  |
| EDTA | 1 mM, 66% residual activity | Thermothelomyces thermophilus | |
| Fe3+ | 1 mM, 62% residual activity | Thermothelomyces thermophilus | |
| Mg2+ | 1 mM, 54% residual activity | Thermothelomyces thermophilus | |
| Pb2+ | 1 mM, 30% residual activity | Thermothelomyces thermophilus | |
| phenylmethylsulfonyl fluoride | 1 mM, 60% residual activity | Thermothelomyces thermophilus | |
| SDS | 0.1%, 56% residual activity | Thermothelomyces thermophilus | |
| Zn2+ | 1 mM, 64% residual activity | Thermothelomyces thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethylcellulose | Km value 5 mg/ml, pH 10, 50°C | Thermothelomyces thermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
gel fitration | Thermothelomyces thermophilus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 5%, 75% residual activity | Thermothelomyces thermophilus |
| Butanol | 5%, 75% residual activity | Thermothelomyces thermophilus |
| Ethanol | 5%, 70% residual activity | Thermothelomyces thermophilus |
| Glycerol | 5%, 85% residual activity | Thermothelomyces thermophilus |
| hexane | 5%, 85% residual activity | Thermothelomyces thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces thermophilus | - |
- |
- |
| Thermothelomyces thermophilus BJA | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Thermothelomyces thermophilus | cellobiose + cellotriose + cellotetraose + cellopentaose | - |
? | |
| carboxymethylcellulose + H2O | - |
Thermothelomyces thermophilus BJA | cellobiose + cellotriose + cellotetraose + cellopentaose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, SDS-PAGE | Thermothelomyces thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EGL | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Thermothelomyces thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
half-life 60 min | Thermothelomyces thermophilus |
| 100 | - |
half-life 15 min | Thermothelomyces thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1020 | - |
carboxymethylcellulose | pH 10, 50°C | Thermothelomyces thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Thermothelomyces thermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 12 | 3 h, about 70% residual activity | Thermothelomyces thermophilus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethylcellulose | kcat/Km value 204 ml/mg/s | Thermothelomyces thermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
