Application | Comment | Organism |
---|---|---|
degradation | enzyme releases high amounts of reducing sugars from wheat bran and corn cobs, being a useful biocatalyst for producing bioethanol and fine chemicals from agroresidues | Thermothelomyces thermophilus |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Thermothelomyces thermophilus |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure. Residues E234 and E344 display a catalytically important role | Thermothelomyces thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 62% residual activity | Thermothelomyces thermophilus | |
EDTA | 1 mM, 66% residual activity | Thermothelomyces thermophilus | |
Fe3+ | 1 mM, 62% residual activity | Thermothelomyces thermophilus | |
Mg2+ | 1 mM, 54% residual activity | Thermothelomyces thermophilus | |
Pb2+ | 1 mM, 30% residual activity | Thermothelomyces thermophilus | |
phenylmethylsulfonyl fluoride | 1 mM, 60% residual activity | Thermothelomyces thermophilus | |
SDS | 0.1%, 56% residual activity | Thermothelomyces thermophilus | |
Zn2+ | 1 mM, 64% residual activity | Thermothelomyces thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carboxymethylcellulose | Km value 5 mg/ml, pH 10, 50°C | Thermothelomyces thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel fitration | Thermothelomyces thermophilus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 5%, 75% residual activity | Thermothelomyces thermophilus |
Butanol | 5%, 75% residual activity | Thermothelomyces thermophilus |
Ethanol | 5%, 70% residual activity | Thermothelomyces thermophilus |
Glycerol | 5%, 85% residual activity | Thermothelomyces thermophilus |
hexane | 5%, 85% residual activity | Thermothelomyces thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces thermophilus | - |
- |
- |
Thermothelomyces thermophilus BJA | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
Thermothelomyces thermophilus | cellobiose + cellotriose + cellotetraose + cellopentaose | - |
? | |
carboxymethylcellulose + H2O | - |
Thermothelomyces thermophilus BJA | cellobiose + cellotriose + cellotetraose + cellopentaose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE | Thermothelomyces thermophilus |
Synonyms | Comment | Organism |
---|---|---|
EGL | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Thermothelomyces thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life 60 min | Thermothelomyces thermophilus |
100 | - |
half-life 15 min | Thermothelomyces thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1020 | - |
carboxymethylcellulose | pH 10, 50°C | Thermothelomyces thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Thermothelomyces thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 12 | 3 h, about 70% residual activity | Thermothelomyces thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carboxymethylcellulose | kcat/Km value 204 ml/mg/s | Thermothelomyces thermophilus |