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Literature summary for 3.2.1.4 extracted from

  • Datta, S.; Holmes, B.; Park, J.; Chen, Z.; Dibble, D.; Hadi, M.; Blanch, H.; Simmons, B.; Sapra, R.
    Ionic liquid tolerant hyperthermophilic cellulases for biomass pretreatment and hydrolysis (2010), Green Chem., 12, 338-345 .
No PubMed abstract available

Application

Application Comment Organism
biofuel production potential of using the ionic liquids-tolerant extremophilic cellulases for hydrolysis of ionic liquids-pretreated lignocellulosic biomass, for biofuel production Thermotoga maritima
biofuel production potential of using the ionic liquids-tolerant extremophilic cellulases for hydrolysis of ionic liquids-pretreated lignocellulosic biomass, for biofuel production Pyrococcus horikoshii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima
expression in Escherichia coli Pyrococcus horikoshii

General Stability

General Stability Organism
under optimum conditions the enzyme shows significantly higher ionic liquid 1-ethyl-3-methylimidazolium acetate tolerance than the Trichoderma viride cellulase Thermotoga maritima
under optimum conditions the enzyme shows significantly higher ionic liquid 1-ethyl-3-methylimidazolium acetate tolerance than the Trichoderma viride cellulase Pyrococcus horikoshii

Organic Solvent Stability

Organic Solvent Comment Organism
1-ethyl-3-methylimidazolium acetate under optimum conditions the enzyme shows significantly higher 1-ethyl-3-methylimidazolium acetate tolerance than the Trichoderma viride cellulase Thermotoga maritima
1-ethyl-3-methylimidazolium acetate under optimum conditions the enzyme shows significantly higher 1-ethyl-3-methylimidazolium acetate tolerance than the Trichoderma viride cellulase Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-
Pyrococcus horikoshii OT-3
-
-
-
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermotoga maritima
-
Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
Thermotoga maritima ?
-
?
avicel + H2O
-
Pyrococcus horikoshii ?
-
?
avicel + H2O
-
Pyrococcus horikoshii OT-3 ?
-
?
carboxymethyl cellulose + H2O
-
Thermotoga maritima ?
-
?
carboxymethyl cellulose + H2O
-
Pyrococcus horikoshii ?
-
?
carboxymethyl cellulose + H2O
-
Pyrococcus horikoshii OT-3 ?
-
?

Synonyms

Synonyms Comment Organism
Cel5A
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermotoga maritima
85
-
assay at Pyrococcus horikoshii
97
-
-
Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
Thermotoga maritima
5.6
-
assay at Pyrococcus horikoshii
6.4
-
-
Pyrococcus horikoshii