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Literature summary for 3.2.1.4 extracted from

  • Huang, X.M.; Li, Q.Q.; Chen, X.L.; Fan, J.X.; Xu, X.H.; Sun, X.D.; Li, D.Y.; Zhao, H.X.
    Expression and characteristics of an endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae (2017), Appl. Biochem. Biotechnol., 182, 1158-1170 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Trichoderma atroviride

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Trichoderma atroviride
K+
-
 Trichoderma atroviride
Na+
-
 Trichoderma atroviride
Zn2+
-
 Trichoderma atroviride

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 raffinose Km value 0.011 mg/ml, pH 5.0, 60°C Trichoderma atroviride
additional information
-
 Avicel Km value 0.0122 mg/ml, pH 5.0, 60°C Trichoderma atroviride
additional information
-
 carboxymethylcellulose Km value 0.014 mg/ml, pH 5.0, 60°C Trichoderma atroviride
additional information
-
 cellobiose Km value 0.025 mg/ml, pH 5.0, 60°C Trichoderma atroviride

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.75 mM, 1.4fold stimulation Trichoderma atroviride
Mg2+
-
 Trichoderma atroviride
Mn2+
-
 Trichoderma atroviride

Organism

Organism UniProt Comment Textmining
Trichoderma atroviride A0A0C5H8Q7
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a signal peptide of 21 amino acids Trichoderma atroviride

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Trichoderma atroviride

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
119.9
-
 pH 5.0, 60°C Trichoderma atroviride

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
 Trichoderma atroviride ?
-
 ?
carboxymethylcellulose + H2O
-
 Trichoderma atroviride ?
-
 ?
cellobiose + H2O
-
 Trichoderma atroviride 2 D-glucose
-
 ?
raffinose + H2O
-
 Trichoderma atroviride ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44230, plus a signal peptide of 21 amino acids, calculated Trichoderma atroviride

Synonyms

Synonyms Comment Organism
EGII
-
 Trichoderma atroviride

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Trichoderma atroviride

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, stable Trichoderma atroviride

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.091
-
 Avicel pH 5.0, 60°C Trichoderma atroviride
0.092
-
 raffinose pH 5.0, 60°C Trichoderma atroviride
0.094
-
 carboxymethylcellulose pH 5.0, 60°C Trichoderma atroviride
0.095
-
 cellobiose pH 5.0, 60°C Trichoderma atroviride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Trichoderma atroviride

pH Range

pH Minimum pH Maximum Comment Organism
3 6
-
 Trichoderma atroviride

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma atroviride calculated
-
 5

Expression

Organism Comment Expression
Trichoderma atroviride expression is repressed by glucose down
Trichoderma atroviride expression is induced by microcrystalline cellulose, bran, carboxymethyl cellulose, rice straw, and corn stalk up